Overexpression of SPAG9 in human gastric cancer is correlated with poor prognosis

被引：0
作者：
Zhi-Feng Miao
Zhen-Ning Wang
Ting-Ting Zhao
Ying-Ying Xu
Jian-Hua Wu
Xing-Yu Liu
Hao Xu
Yi You
Hui-Mian Xu
机构：
[1] the First Affiliated Hospital of China Medical University,Department of Surgical Oncology
[2] the First Affiliated Hospital of China Medical University,Department of Breast Surgery
来源：
Virchows Archiv | 2015年 / 467卷
关键词：
SPAG9; Gastric cancer; Proliferation; Chemoresistance;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Sperm associated antigen 9 (SPAG9) protein has been found to play an important role in cancer progression but the involved mechanisms are still obscure. Its clinical significance in human gastric cancers remains unexplored. In the present study, SPAG9 expression was analyzed in 147 gastric cancer specimens. We observed weak staining in normal gastric mucosa and positive staining in 65 out of 147 (44.2 %) cancer samples. Overexpression of SPAG9 correlated with local invasion (p = 0.0101), lymph node metastasis (p = 0.0488), TNM stage (p = 0.0002), and relapse (p = 0.0018). Importantly, SPAG9 overexpression correlated with poor overall survival (p = 0.0008). Furthermore, we performed siRNA knockdown of SPAG9 in HGC-27 cells with high endogenous expression and transfected SPAG9 plasmid in SGC-7901 cell line with low endogenous level. SPAG9 overexpression promoted while its depletion inhibited cell proliferation, cell cycle transition, and invasive cell growth. SPAG9 overxpression also increased chemoresistance to 5-fluorouracil (5-FU) in SGC-7901 cells. Further analysis showed that SPAG9 knockdown downregulated and its overexpression upregulated cyclin D1, MMP9, and p-p38 expression. In conclusion, SPAG9 overexpression in gastric cancer correlates with poor prognosis and contributes to gastric cancer cell proliferation, invasion, and chemoresistance. SPAG9 promotes gastric cancer invasion, possibly through p38-MMP9 signaling pathways.
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页码：525 / 533
页数：8
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