Advances in cryopreservation of in vitro-derived propagules: technologies and explant sources

被引:0
作者
Min-Rui Wang
Maurizio Lambardi
Florent Engelmann
Ranjith Pathirana
Bart Panis
Gayle M. Volk
Qiao-Chun Wang
机构
[1] Northwest A&F University,State Key Laboratory of Crop Stress Biology in Arid Region, College of Horticulture
[2] National Research Council (CNR),CNR
[3] IRD Representation in Benin,IBE, Institute of Bio
[4] Résidence Les Cocotiers,Economy
[5] New Zealand Institute for Plant & Food Research Ltd,Biotechnics
[6] K.U. Leuven,undefined
[7] Bioversity International,undefined
[8] National Laboratory for Genetic Resources Preservation,undefined
来源
Plant Cell, Tissue and Organ Culture (PCTOC) | 2021年 / 144卷
关键词
Cryopreservation; Cryo-plate; Cryo-mesh; Droplet-vitrification; Microtubers; Shoot tips;
D O I
暂无
中图分类号
学科分类号
摘要
Genetic improvements in plant breeding are dependent upon having access to novel plant genetic resources that are available in plant genebanks. Many crops that are vegetatively-propagated are maintained as plants in the field or greenhouse, making them vulnerable to biotic and abiotic threats. Increasingly, plant genebanks are using cryopreservation technologies to secure vegetatively propagated collections at secondary locations. Droplet vitrification and cryo-plate cryopreservation methods have been used to successfully cryopreserve the shoot tips of many plant species. New propagule types, including small leaf square-bearing adventitious buds, stem disc-bearing adventitious buds, microtubers and rhizome buds are alternative explants for use in cryopreservation. This review describes new technologies for in-vitro based cryopreservation systems that have advanced the field of plant cryopreservation. Future advances will allow even more diverse germplasm to be successfully preserved in cryobanks.
引用
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页码:7 / 20
页数:13
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