Effects of heavy metals on transcription and enzyme activity of Na+/K+-ATPase in the monogonont rotifer, Brachionus koreanus

被引：8

作者：

Kim H. ^{[1
]}

Lim B. ^{[1
]}

Kim B.-D. ^{[1
]}

Lee Y.-M. ^{[1
]}

机构：

[1] Department of Life Science, College of Natural Sciences, Sangmyung University, Seoul

来源：

Toxicology and Environmental Health Sciences | 2016年 / 8卷 / 2期

关键词：

Brachionus koreanus; Heavy metals; Na[!sup]+[!/sup]/K[!sup]+[!/sup]-ATPase; Quantitative real-time RT-PCR; Rotifer;

D O I：

10.1007/s13530-016-0270-4

中图分类号：

学科分类号：

摘要：

Heavy metals can lead to osmotic stress by disrupting the regulation of sodium ion in aquatic organisms. In this study, gene expression patterns and enzymatic activities of Na+/K+-ATPase in the monogonont rotifer, Brachionus koreanus were measured after exposure to different Cd (7.5, 15, and 30 mg/L), and Pb (0.1, 0.5, and 1.0 mg/L), respectively. As results, a significant increase in Bk Na+/K+-ATPase activity was observed after exposure to Cd and Pb in a concentration-dependent manner. BkNa+/K+-ATPase mRNA level was significantly upregulated in the Cd-exposed group, whereas its level was reduced in the Pb-exposed group. These findings indicate that heavy metals could induce osmotic stress in B. koreanus, and Na+/K+-ATPase may be involved in cellular ho-meostasis in response to heavy metal exposure. This study is helpful for the understanding of the molecular mode of action of B. koreanus in response to heavy metals. © 2016, Korean Society of Environmental Risk Assessment and Health Science and Springer Science+Business Media Dordrecht.

引用

页码：128 / 134

页数：6

共 50 条

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