High-throughput identification of proteins with AMPylation using self-assembled human protein (NAPPA) microarrays

被引：0
|
作者：
Xiaobo Yu
Joshua LaBaer
机构：
[1] The Virginia G. Piper Center for Personalized Diagnostics,
[2] Biodesign Institute,undefined
[3] Arizona State University,undefined
来源：
Nature Protocols | 2015年 / 10卷
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学科分类号：
摘要：
This protocol allows the identification of AMPylated and auto-AMPylated proteins using high-density protein microarrays fabricated using NAPPA technology. Target protein modification is determined via copper-catalyzed azide-alkyne cycloaddition.
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页码：756 / 767
页数：11
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