Ganzfeld ERG in zebrafish larvae

被引：28

作者：

Seeliger M.W. ^{[1
]}

Rilk A. ^{[1
]}

Neuhauss S.C.F. ^{[2
,3
]}

机构：

[1] Dept. II, University Eye Hospital, Tübingen D-72076

[2] Max-Planck-Institut für Entwicklungsbiologie Abt. i, Tübingen

[3] Brain Research Institute, ETH Zurich, Zurich CH-8057

来源：

Documenta Ophthalmologica | 2002年 / 104卷 / 1期

关键词：

Animal model; Electrophysiology; ERG; Zebrafish;

D O I：

10.1023/A:1014454927931

中图分类号：

学科分类号：

摘要：

In developmental biology, zebrafish are widely used to study the impact of mutations. The fast pace of development allows for a definitive morphological evaluation of the phenotype usually 5 days post fertilization (dpf). At that age, a functional analysis is already feasible using electroretinographic (ERG) methods. Corneal Ganzfeld ERGs were recorded with a glass microelectrode in anaesthetized, dark-adapted larvae aged 5 dpf, using a platinum wire beneath a moist paper towel as reference. ERG protocols included flash, flicker, and ON/OFF stimuli, both under scotopic and photopic conditions. Repetitive, isoluminant stimuli were used to assess the dynamic effect of pharmacological agents on the ERG. Single flash, flicker, and ON/OFF responses had adequately matured at this point to be informative. Typical signs of the cone dominance were the small scotopic a-wave and the large OFF responses. The analysis of consecutive single traces was possible because of the lack of EKG, breathing, and blink artefacts. After application of APB, which selectively blocks the ON channel via the mGluR6 receptor, the successive loss of the b-wave could be observed, which was quite different from the deterioration of the ERG after a circulatory arrest. The above techniques allowed to reliably obtain Ganzfeld ERGs in larvae aged 5 dpf. This underlines the important role of the zebrafish as a model for the functional analysis of mutations disrupting the visual system.

引用

页码：57 / 68

页数：11

共 35 条

[21]

Li L., Dowling J.E., A dominant form of inherited retinal degeneration caused by a non-photoreceptor cell-specific mutation, Proc Natl Acad Sci USA, 94, pp. 11645-11650, (1997)

[22]

Li L., Dowling J.E., Effects of dopamine depletion on visual sensitivity of zebrafish, J Neurosci, 20, pp. 1893-1903, (2000)

[23]

Mullins M.C., Hammerschmidt M., Haffter P., Nusslein-Volhard C., Large-scale mutagenesis in the zebrafish: In search of genes controlling development in a vertebrate, Curr Biol, 4, pp. 189-202, (1994)

[24]

Sieving P.A., Photopic ON- and OFF-pathway abnormalities in retinal dystrophies, Trans Am Ophthalmol Soc, 91, pp. 701-773, (1993)

[25]

Seeliger M.W., Neuhauss S.C.F., Kohler K., Zrenner E., Ganzfeld-electroretinography in the zebrafish, Invest Ophthalmol Vis Sci [Suppl], 39, (1998)

[26]

Saszik S., Bilotta J., The effects of temperature on the dark-adapted spectral sensitivity function of the adult zebrafish, Vision Res, 39, pp. 1051-1058, (1999)

[27]

Biel M., Seeliger M.W., Pfeifer A., Kohler K., Gerstner A., Ludwig A., Jaissle G., Fauser S., Zrenner E., Hofmann F., Selective loss of cone function in mice lacking the cyclic nucleotide-gated channel CNG3, Proc Natl Acad Sci USA, 96, pp. 7553-7557, (1999)

[28]

Peachey N.S., Alexander K.R., Fishman G.A., The luminance-response function of the dark-adapted human electroretinogram, Vision Res, 29, pp. 263-270, (1989)

[29]

Vihtelic T.S., Doro C.J., Hyde D.R., Cloning and characterization of six zebrafish photoreceptor opsin cDNAs and immunolocalization of their corresponding proteins, Vis Neurosci, 16, pp. 571-585, (1999)

[30]

Raymond P.A., Barthel L.K., Curran G.A., Developmental patterning of rod and cone photoreceptors in embryonic zebrafish, J Comp Neurol, 359, pp. 537-550, (1995)

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