Ganzfeld ERG in zebrafish larvae

被引：28

作者：

Seeliger M.W. ^{[1
]}

Rilk A. ^{[1
]}

Neuhauss S.C.F. ^{[2
,3
]}

机构：

[1] Dept. II, University Eye Hospital, Tübingen D-72076

[2] Max-Planck-Institut für Entwicklungsbiologie Abt. i, Tübingen

[3] Brain Research Institute, ETH Zurich, Zurich CH-8057

来源：

Documenta Ophthalmologica | 2002年 / 104卷 / 1期

关键词：

Animal model; Electrophysiology; ERG; Zebrafish;

D O I：

10.1023/A:1014454927931

中图分类号：

学科分类号：

摘要：

In developmental biology, zebrafish are widely used to study the impact of mutations. The fast pace of development allows for a definitive morphological evaluation of the phenotype usually 5 days post fertilization (dpf). At that age, a functional analysis is already feasible using electroretinographic (ERG) methods. Corneal Ganzfeld ERGs were recorded with a glass microelectrode in anaesthetized, dark-adapted larvae aged 5 dpf, using a platinum wire beneath a moist paper towel as reference. ERG protocols included flash, flicker, and ON/OFF stimuli, both under scotopic and photopic conditions. Repetitive, isoluminant stimuli were used to assess the dynamic effect of pharmacological agents on the ERG. Single flash, flicker, and ON/OFF responses had adequately matured at this point to be informative. Typical signs of the cone dominance were the small scotopic a-wave and the large OFF responses. The analysis of consecutive single traces was possible because of the lack of EKG, breathing, and blink artefacts. After application of APB, which selectively blocks the ON channel via the mGluR6 receptor, the successive loss of the b-wave could be observed, which was quite different from the deterioration of the ERG after a circulatory arrest. The above techniques allowed to reliably obtain Ganzfeld ERGs in larvae aged 5 dpf. This underlines the important role of the zebrafish as a model for the functional analysis of mutations disrupting the visual system.

引用

页码：57 / 68

页数：11

共 35 条

[1]

Driever W., Solnica-Krezel L., Schier A.F., Neuhauss S.C., Malicki J., Stemple D.L., Stainier D.Y., Zwartkruis F., Abdelilah S., Rangini Z., Belak J., Boggs C., A genetic screen for mutations affecting embryogenesis in zebrafish, Development, 123, pp. 37-46, (1996)

[2]

Haffter P., Granato M., Brand M., Mullins M.C., Hammerschmidt M., Kane D.A., Odenthal J., Van Eeden F.J., Jiang Y.J., Heisenberg C.P., Kelsh R.N., Furutani-Seiki M., Vogelsang E., Beuchle D., Schach U., Fabian C., Nusslein-Volhard C., The identification of genes with unique and essential functions in the development of the zebrafish, Danio rerio, Development, 123, pp. 1-36, (1996)

[3]

Malicki J., Neuhauss S.C., Schier A.F., Solnica-Krezel L., Stemple D.L., Stainier D.Y., Abdelilah S., Zwartkruis F., Rangini Z., Driever W., Mutations affecting development of the zebrafish retina, Development, 123, pp. 263-273, (1996)

[4]

Fadool J.M., Brockerhoff S.E., Hyatt G.A., Dowling J.E., Mutations affecting eye morphology in the developing zebrafish (Danio rerio), Dev Genet, 20, pp. 288-295, (1997)

[5]

Hisaoka K.K., Battle H.I., The normal developmental stages of the zebrafish, Brachydanio rerio (Hamilton-Buchanan), J Morphol, 102, pp. 311-323, (1958)

[6]

Branchek T., Bremiller R., The development of photoreceptors in the zebrafish, Brachydanio rerio. I. Structure, J Comp Neurol, 224, pp. 107-115, (1984)

[7]

Kljavin I.J., Early development of photoreceptors in the ventral retina of the zebrafish embryo, J Comp Neurol, 260, pp. 461-471, (1987)

[8]

Larison K.D., Bremiller R., Early onset of phenotype and cell patterning in the embryonic zebrafish retina, Development, 109, pp. 567-576, (1990)

[9]

Schmitt E.A., Dowling J.E., Early eye morphogenesis in the zebrafish, Brachydanio rerio, J Comp Neurol, 344, pp. 532-542, (1994)

[10]

Schmitt E.A., Dowling J.E., Comparison of topographical patterns of ganglion and photoreceptor cell differentiation in the retina of the zebrafish, Danio rerio, J Comp Neurol, 371, pp. 222-234, (1996)

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