Development of polymorphic microsatellite markers for the North American porcupine, Erethizon dorsatum, using paired-end Illumina sequencing

被引：0

作者：

Erika L. Barthelmess

Cara N. Love

Kenneth L. Jones

Stacey L. Lance

机构：

[1] St. Lawrence University,Biology Department

[2] University of Georgia,Savannah River Ecology Laboratory

[3] University of Colorado School of Medicine,Department of Biochemistry and Molecular Genetics

来源：

Conservation Genetics Resources | 2013年 / 5卷

关键词：

Porcupine; Illumina; Microsatellite; PAL_FINDER; PCR primers; SSR;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

We isolated and characterized a total of 19 microsatellite loci from the North American porcupine, Erethizon dorsatum. Loci were screened in 22 individuals from St. Lawrence and Franklin Counties in northern New York State. The number of alleles per locus ranged from 4 to 14, observed heterozygosity ranged from 0.316 to 1.000, and the probability of identity values ranged from 0.028 to 0.243. These new loci will provide tools for examination of the effects of habitat fragmentation, for instance by roads and other infrastructure, on gene flow and population subdivision in this species.

引用

页码：925 / 927

页数：2

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