13C, 15N and 1H resonance assignments of receiver domain of ethylene receptor ETR1

被引：0
作者：
Yi-Lin Hung
Yi-Jan Lin
Shih-Che Sue
机构：
[1] National Tsing Hua University,Institute of Bioinformatics and Structural Biology
[2] National Tsing Hua University,Department of Life Science
[3] Kaohsiung Medical University,Graduate Institute of Natural Products and Center of Excellence for Environmental Medicine
来源：
Biomolecular NMR Assignments | 2015年 / 9卷
关键词：
Ethylene; Ethylene receptor; Two-component system; Receiver domain;
D O I：
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中图分类号：
学科分类号：
摘要：
Ethylene plays versatile functions in regulating plant physiology. Although the high affinity ethylene receptor and its downstream regulators have been identified, the molecular recognition of the receptor interacting domains remains to be established. It has been speculated that the cytoplasmic signaling of the ethylene receptor is a two-component regulatory system involving the conserved receiver domain (RD). Here, we report the NMR chemical shift assignments for RD from Arabidopsis thaliana ethylene receptor ETR1. Nearly complete backbone and side-chain assignments were achieved at pH 6.0 and 25 °C. The assignments and backbone dynamics revealed the secondary structure and showed that ETR1-RD is a monomer in solution. These results will make it possible to monitor downstream binding partners and elucidates our understanding of phosphotransfer in the plant two-component regulatory system in the ethylene signaling pathway.
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页码：119 / 122
页数：3
相关论文
共 36 条
  • [1] Binder BM(2008)The ethylene receptors: complex perception for a simple gas Plant Sci 175 8-17
  • [2] Bleecker AB(1998)The ethylene–receptor family from Arabidopsis: structure and function Philos Trans R Soc Lond 353 1405-1412
  • [3] Bleecker AB(2000)Ethylene: a gaseous signal molecule in plants Annu Rev Cell Dev Biol 16 1-18
  • [4] Kende H(1993)Arabidopsis ethylene-response gene ETR1: similarity of product to two-component regulators Science 262 539-544
  • [5] Chang C(2002)Localization of the ethylene receptor ETR1 to the endoplasmic reticulum of Arabidopsis J Biol Chem 277 19861-19866
  • [6] Kwok SF(1998)Association of the Arabidopsis CTR1 Raf-like kinase with the ETR1 and ERS ethylene receptors Proc Natl Acad Sci 95 5401-5406
  • [7] Bleecker AB(1995)NMRPipe: a multidimensional spectral processing system based on UNIX pipes J Biomol NMR 6 277-293
  • [8] Meyerowitz EM(2007)Ethylene receptors: ethylene perception and signal transduction J Plant Growth Regul 26 118-130
  • [9] Chen Y-F(2003)Biochemical and functional analysis of CTR1, a protein kinase that negatively regulates ethylene signaling in Arabidopsis Plant J 33 221-233
  • [10] Randlett MD(1999)The structure of the signal receiver domain of the Structure 7 1547-1556
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