Identification and testing of reference genes for qRT-PCR analysis during pear fruit development

被引：0
作者：
Guoming Wang
Zhihua Guo
Xueping Wang
Sophia Lee Guan
Hongru Gao
Kaijie Qi
Chao Gu
Shaoling Zhang
机构：
[1] Nanjing Agricultural University,Centre of Pear Engineering Technology Research, State Key Laboratory of Crop Genetics and Germplasm Enhancement
[2] University of Maryland,College of Computer, Mathematical, and Natural Sciences
来源：
Biologia | 2022年 / 77卷
关键词：
Fruit development; Pear; qRT-PCR; Reference genes;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Quantitative real-time PCR (qRT-PCR) is currently one of the most reliable and improved tools for analyzing gene expression. Various studies have shown that housekeeping genes vary with cultivars, tissues and treatment. Reliable and stable reference genes were necessarily identified and evaluated according to different experimental requirements. In this study, 10 candidate reference genes were initially screened based on transcriptome sequencing data of four pear fruit development stages for three pear cultivars, including a candidate housekeeping gene PbrTUB. Furthermore, we ranked the expression stability of 10 candidate reference genes using GeNorm, NormFinder, BestKeeper and ReFinder algorithms. The results showed that Pbr028511, Pbr038418 and Pbr041114 were the most stable reference genes in Cuiguan, Housui and Xueqing fruit, respectively. Taken together, these results serve as a useful reference for gene function investigations and molecular mechanism studies in fruit development and ripening for various pear cultivars.
引用
收藏
页码：2763 / 2777
页数：14
相关论文
共 348 条
[1]  
Andersen CL(2004)Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets Cancer Res 64 5245-5250
[2]  
Jensen JL(2005)Quantitative real-time RT-PCR - a perspective J Mol Endocrinol 34 597-601
[3]  
Ørntoft TF(2011)Validation of reference genes for RT-qPCR studies of gene expression in banana fruit under different experimental conditions Planta 234 377-390
[4]  
Bustin SA(2015)Identification and testing of reference genes for gene expression analysis in pollen of Pyrus bretschneideri Sci Hortic 190 43-56
[5]  
Benes V(2018)Phosphatidic acid counteracts S-RNase signaling in pollen by stabilizing the actin cytoskeleton Plant Cell 30 1023-1039
[6]  
Nolan T(2020)Genome-wide identification and validation of optimal reference genes for gene expression normalization in pear peel Plant Growth Regul 92 249-261
[7]  
Pfaffl MW(2020)Genome-wide identification and testing of superior reference genes for transcript normalization during analyses of flesh development in Asian pear cultivars Sci Hortic 271 109459-14
[8]  
Chen L(2021)Genome-wide identification of ubiquitin proteasome subunits as superior reference genes for transcript normalization during receptacle development in strawberry cultivars BMC Genomics 22 1-230
[9]  
Zhong HY(2011)Selection and validation of reference genes for quantitative real-time PCR in buckwheat (Fagopyrum esculentum) based on transcriptome sequence data PLoS One 6 e19434-6077
[10]  
Kuang JF(2010)How to do successful gene expression analysis using real-time PCR Methods 50 227-12
12345678910