The Deoxymiroestrol and Isoflavonoid Production and Their Elicitation of Cell Suspension Cultures of Pueraria candollei var. mirifica: from Shake Flask to Bioreactor

被引:0
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作者
Orapin Udomsin
Gorawit Yusakul
Tharita Kitisripanya
Thaweesak Juengwatanatrakul
Waraporn Putalun
机构
[1] Khon Kaen University,Faculty of Pharmaceutical Sciences
[2] National Research University-Khon Kaen University,Research Group for Faculty of Pharmaceutical Activities of Natural Products Using Pharmaceutical Biotechnology (PANPB)
[3] Walailak University,Drug and Cosmetics Excellence Center
[4] Walailak University,School of Pharmacy
[5] Mahidol University,Department of Pharmacognosy, Faculty of Pharmacy
[6] Ubon Ratchathani University,Faculty of Pharmaceutical Sciences
来源
Applied Biochemistry and Biotechnology | 2020年 / 190卷
关键词
var. ; Cell suspension; Bioreactor; Deoxymiroestrol; Isoflavonoid;
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学科分类号
摘要
To address the high demand for Pueraria candollei var. mirifica (PM) used as the active ingredient in health products and its difficulty to cultivate in the field, the growth and production of deoxymiroestrol (DME) and isoflavonoid (ISF) phytoestrogens in PM cell suspensions were studied. In a 125-mL shake flask, the cell suspension produced DME [78.7 ± 8.79–116 ± 18.2 μg/g dry weight (DW)] and ISF (140 ± 6.83–548 ± 18.5 μg/g DW), which are the predominant ISF glycosides. While ISF aglycones accumulated in the PM cell suspension cultured in the airlift bioreactor. The DME content was increased to 976 ± 79.6 μg/g DW when the PM cell suspension was cultured in the 5-L scale bioreactor. The production of DME and ISF was enhanced by elicitors including methyl jasmonate (MJ), yeast extract (YE), and chitosan (CHI). MJ produced the highest induction of DME accumulation, while ISF accumulation was the highest with YE treatment. Analysis of catalase activity implied that the elicitors enhanced ROS production, which resulted in the enhancement of DME and ISF production and accumulation in PM cell suspension cultures. PM cell suspension culture is a promising source of beneficial PM phytoestrogens that exhibit bioactivity that may useful for the treatment of menopausal symptoms.
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页码:57 / 72
页数:15
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