Development, expansion and maturation of hematopoietic stem cells in mouse embryo

被引:0
作者
Atsushi Miyajima
Ken-ichi Minehata
Masaki Takeuchi
机构
[1] The University of Tokyo,Institute of Molecular and Cellular Biosciences
来源
International Journal of Hematology | 2002年 / 76卷
关键词
D O I
暂无
中图分类号
学科分类号
摘要
During embryonic development, hematopoiesis occurs in different tissues. Primitive hematopoiesis first occurs in the yolk sac and definitive hematopoietic stem cells (HSCs) arise in the aorta/gonad/mesonephros (AGM) region. Fetal liver then functions as the major hematopoietic organ from the mid to late gestation, before hematopoiesis starts in the bone marrow. We have developed a primary culture system of mouse AGM cells which produces both hematopoietic cells and endothelial cells and found that hemangioblasts, the common precursor of hematopotieic and endothelial cells, are enriched in the cell population which express podocalyxin like protein 1, a CD34-related molecule, but lacks CD45 in the AGM region. We also developed a culture system of E14.5 fetal hepatic cells which support hematopoiesis. AGM-derived HSCs proliferated most vigorously in the presence of such fetal hepatic cells, however HSCs derived from later stages of development proliferated less efficiently in the same co-culture system, suggesting that the proliferation potential of HSCs declines along with development. While AGM-derived HSCs poorly engrafted in the bone marrow of irradiated adult mice, co-culture of AGM-derived HSCs with fetal hepatic cells dramatically increased the bone marrow engraftment. These results indicate that fetal hepatic cells alter the characteristics of HSCs. Interestingly, Oncostatin M (OSM) strongly induced differentiation of immature hepatocytes to metabolically active mature hepatocytes, but it simultaneously reduced their ability to support hematopoiesis. As OSM is produced by hematopoietic cells in the fetal liver, it is likely that OSM plays a role for coordinating the development of hematopoietic cells and the liver. In conclusion, our in vitro system provides a means to study the molecular basis of development, expansion and maturation of HSCs.
引用
收藏
页码:153 / 153
相关论文
共 50 条
  • [31] Analysis of the Spatiotemporal Development of Hematopoietic Stem and Progenitor Cells in the Early Human Embryo
    Easterbrook, Jennifer
    Rybtsov, Stanislav
    Gordon-Keylock, Sabrina
    Ivanovs, Andrejs
    Taoudi, Samir
    Anderson, Richard A.
    Medvinsky, Alexander
    [J]. STEM CELL REPORTS, 2019, 12 (05): : 1056 - 1068
  • [32] Hemogenic Endothelial Cells Can Transition to Hematopoietic Stem Cells through a B-1 Lymphocyte-Biased State during Maturation in the Mouse Embryo
    Kobayashi, Michihiro
    Tarnawsky, Stefan P.
    Wei, Haichao
    Mishra, Akansha
    Portilho, Nathalia Azevedo
    Wenzel, Pamela
    Davis, Brian
    Wu, Jiaqian
    Hadland, Brandon
    Yoshimoto, Momoko
    [J]. STEM CELL REPORTS, 2019, 13 (01): : 21 - 30
  • [33] MOUSE HEMATOPOIETIC STEM-CELLS
    SPANGRUDE, GJ
    SMITH, L
    UCHIDA, N
    IKUTA, K
    HEIMFELD, S
    FRIEDMAN, J
    WEISSMAN, IL
    [J]. BLOOD, 1991, 78 (06) : 1395 - 1402
  • [34] Role of estrogen receptor α in hematopoietic stem cell development and B lymphocyte maturation in the male mouse
    Thurmond, TS
    Murante, FG
    Staples, JE
    Silverstone, AE
    Korach, KS
    Gasiewicz, TA
    [J]. ENDOCRINOLOGY, 2000, 141 (07) : 2309 - 2318
  • [35] OVARIAN SUPPORT CELLS DERIVED FROM HUMAN INDUCED PLURIPOTENT STEM CELLS ALLOW IN VITRO MATURATION OF OOCYTES AND EMBRYO DEVELOPMENT IN A MOUSE MODEL
    Marchante, Maria
    Potts, Kathryn S.
    Mestres, Enric
    Acacio, Monica
    Piechota, Sabrina
    Giovannini, Alexa
    Paulsen, Bruna
    Costa Borges, Nuno
    Kramme, Christian
    [J]. FERTILITY AND STERILITY, 2023, 120 (04) : E45 - E45
  • [36] Development of defined and feeder-free media for the expansion of hematopoietic stem cells
    Jeon, Young-Joo
    Choi, Yong-Soo
    Song, Ji-Hae
    Park, Bu-Young
    Kim, Jong-Il
    Kong, Mi-Eun
    Jeon, Hyung-Jin
    Kim, Dong-Il
    [J]. JOURNAL OF BIOTECHNOLOGY, 2008, 136 : S181 - S181
  • [37] Ex vivo expansion of hematopoietic stem cells
    JingJing Xie
    ChengCheng Zhang
    [J]. Science China Life Sciences, 2015, 58 : 839 - 853
  • [38] In vitro and in vivo expansion of hematopoietic stem cells
    Sauvageau, G
    Iscove, NN
    Humphries, RK
    [J]. ONCOGENE, 2004, 23 (43) : 7223 - 7232
  • [39] In vitro and in vivo expansion of hematopoietic stem cells
    Guy Sauvageau
    Norman N Iscove
    R Keith Humphries
    [J]. Oncogene, 2004, 23 : 7223 - 7232
  • [40] Definitive hematopoietic stem cells first develop within the major arterial regions of the mouse embryo
    de Bruijn, MFTR
    Speck, NA
    Peeters, MCE
    Dzierzak, E
    [J]. EMBO JOURNAL, 2000, 19 (11) : 2465 - 2474