Multiple shoot induction by benzyladenine and complete plant regeneration from seed explants of chickpea (Cicer arietinum L.)

被引：6

作者：

Polisetty R. ^{[1
]}

Paul V. ^{[1
]}

Deveshwar J.J. ^{[1
]}

Khetarpal S. ^{[1
]}

Suresh K. ^{[1
]}

Chandra R. ^{[1
]}

机构：

[1] Division of Plant Physiology, Plant Tissue Culture Laboratory, Indian Agric. Research Institute

来源：

Plant Cell Reports | 1997年 / 16卷 / 8期

关键词：

Benzyladenine; Cicer arietinum; Shoot regeneration;

D O I：

10.1007/BF01142325

中图分类号：

学科分类号：

摘要：

The efficacy of benzyladenine (BA) to induce multiple shoots from seed explants of chickpea (Cicer arietinum L.) was assessed. Shoot differentiation was influenced by the type of seed explant, genotype and concentration of BA. Orientation of the explant also strongly influenced the shoot regeneration response. The optimum BA concentration for shoot/shoot bud regeneration was genotype dependent. Two types of BA-induced response were observed: (1) at less than 7.5 μM BA, direct shoot differentiation (2 to 4-cm-long shoots) was observed within 30 days; (2) at higher BA concentrations (75 100 μM), shoot/shoot bud differentiation was achieved in 45-90 days. A high BA concentration inhibited subsequent rooting of shoots. Roots, however, could be easily induced on shoots derived from < 12.5 μM BA. Following transfer to soil, 80% of the regenerants developed into morphologically normal and fertile plants.

引用

页码：565 / 571

页数：6

共 19 条

[1]

Barna K.S., Wakhlu A.K., Somatic embryogenesis and plant regeneration from callus cultures of chickpea (Cicer arietinum L.), Plant Cell Rep, 12, pp. 521-524, (1993)

[2]

Brandt E.B., Hess D., In vitro regeneration and propagation of chickpea (Cicer arietinum L.) from meristem tips and cotyledonary nodes, In Vitro Cell Dev Biol Plant, 30 P, pp. 75-80, (1994)

[3]

Cheng T.Y., Saka H., Voqui-Dinh T.H., Plant regeneration from soybean cotyledonary node segments in culture, Plant Sci Lett, 19, pp. 91-99, (1980)

[4]

Franklin C.I., Trieu T.N., Gongales R.A., Dixon R.A., Plant regeneration from seedling explants of green bean (Phaseolus vulgaris L.) via organogenesis, Plant Cell Tissue Organ Cult, 24, pp. 199-206, (1991)

[5]

Gambley R.L., Dodd W.A., An in vitro technique for the production de novo of multiple shoots in cotyledon explants of cucumber (Cucumis sativus L.), Plant Cell Tissue Organ Cult, 20, pp. 177-183, (1990)

[6]

Gamborg O.L., Miller R.A., Ojima K., Nutrient requirements for suspension cultures of soybean root cells, Exp Cell Res, 50, pp. 151-158, (1968)

[7]

Gill R., Eapen S., Rao P.S., Morphogenic studies of cultured cotyledons of urd bean (Vigna mungo L. Hepper), J Plant Physiol, 130, pp. 1-5, (1987)

[8]

Gulati A., Jaiwal P.K., Culture conditions affecting plant regeneration from cotyledon of mungbean (Vigna radiata L. Wilczek), Plant Cell Tissue Organ Cult, 23, pp. 1-7, (1990)

[9]

Gulati A., Jaiwal P.K., Plant regeneration from cotyledonary node expiants of mungbean (Vigna radiata L. Wilczek), Plant Cell Rep, 13, pp. 523-527, (1994)

[10]

Malik K.A., Saxena P.K., Regeneration in Phaseolus vulgaris L. High frequency induction of direct shoot formation in intact seedlings by N<sup>6</sup>-benzylaminopurine and thidiazuron, Planta, 186, pp. 384-389, (1992)

← 1 2 →