Simultaneous Determination of Tranexamic Acid and Losartan Potassium in Dosage Formulations and Human Serum by RP-LC

Rapid liquid chromatographic procedure for analytical quality control of pharmaceutical preparations and human serum containing drugs, tranexamic acid together with losartan potassium are proposed, using acetonitrile: water (50:50), adjusting pH to 2.6 with phosphoric acid as a mobile phase, UV detection at 205 nm and propylparaben sodium was used as internal standard. The results obtained showed a good agreement with the declared contents. The method shows good linearity in the range of 40-10,000 ng mL(-1) for tranexamic acid serum concentrations with a correlation coefficient 0.9999 (inter- and intra-day CV < 3.18) and in the range 5-10,000 ng mL(-1) for losartan potassium serum concentrations with a correlation coefficient 0.9999 (inter- and intra-day CV < 3.61). The recovery was > 97.8%. The proposed method may be used for the quantitative analysis of tranexamic acid and losartan potassium alone or in combination from raw materials, in bulk drugs, dosage formulations and in serum.