Single-cell RNA Sequencing in Immunology

被引：15

作者：

Cao, Yirui ^{[1
,2
]}

Qiu, Yue ^{[3
]}

Tu, Guowei ^{[3
]}

Yang, Cheng ^{[1
,2
,4
]}

机构：

[1] Fudan Univ, Zhongshan Hosp, Dept Urol, Shanghai 200032, Peoples R China

[2] Shanghai Key Lab Organ Transplantat, Shanghai 200032, Peoples R China

[3] Fudan Univ, Zhongshan Hosp, Dept Crit Care Med, Shanghai 200032, Peoples R China

[4] Fudan Univ, Fudan Zhangjiang Inst, Shanghai 201203, Peoples R China

来源：

CURRENT GENOMICS | 2020年 / 21卷 / 08期

基金：

中国国家自然科学基金; 国家重点研发计划;

关键词：

Single-cell RNA sequencing; immunology; cancer; autoimmune disease; transplantation; tumors; T-CELLS; PROTEINS; PRECURSORS; MICROGLIA; CHROMATIN; SEQ;

D O I：

10.2174/1389202921999201020203249

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The complex immune system is involved in multiple pathological processes. Single-cell RNA sequencing (scRNA-seq) is able to analyze complex cell mixtures correct to a single cell and single molecule, thus is qualified to analyze immune reactions in several diseases. In recent years, scRNA-seq has been applied in many researching fields and has presented many innovative results. In this review, we intend to provide an overview of single-cell RNA sequencing applications in immunology and a prospect of future directions.

引用

页码：564 / 575

页数：12

共 75 条

[1] Psychrophilic proteases dramatically reduce single-cell RNA-seq artifacts: a molecular atlas of kidney development [J].

Adam, Mike ;

Potter, Andrew S. ;

Potter, S. Steven .

DEVELOPMENT, 2017, 144 (19) :3625-3632

[2]

Ashton J. M., 2020, BIORXIV, DOI [10.1101/2020.07.20.212100, DOI 10.1101/2020.07.20.212100]

[3] Circulating precursors of human CD1c+ and CD141+ dendritic cells [J].

Breton, Gaelle ;

Lee, Jaeyop ;

Zhou, Yu Jerry ;

Schreiber, Joseph J. ;

Keler, Tibor ;

Puhr, Sarah ;

Anandasabapathy, Niroshana ;

Schlesinger, Sarah ;

Caskey, Marina ;

Liu, Kang ;

Nussenzweig, Michel C. .

JOURNAL OF EXPERIMENTAL MEDICINE, 2015, 212 (03) :401-413

[4] Single-cell chromatin accessibility reveals principles of regulatory variation [J].

Buenostro, Jason D. ;

Wu, Beijing ;

Litzenburger, Ulrike M. ;

Ruff, Dave ;

Gonzales, Michael L. ;

Snyder, Michael P. ;

Chang, Howard Y. ;

Greenleaf, William J. .

NATURE, 2015, 523 (7561) :486-U264

[5]

Buenrostro JD, 2013, NAT METHODS, V10, P1213, DOI [10.1038/NMETH.2688, 10.1038/nmeth.2688]

[6] Memory CD4+CCR5+ T cells are abundantly present in the gut of newborn infants to facilitate mother-to-child transmission of HIV-1 [J].

Bunders, Madeleine J. ;

van der Loos, Chris M. ;

Klarenbeek, Paul L. ;

van Hamme, John L. ;

Boer, Kees ;

Wilde, Jim C. H. ;

de Vries, Niek ;

van Lier, Rene A. W. ;

Kootstra, Neeltje ;

Pals, Steven T. ;

Kuijpers, Taco W. .

BLOOD, 2012, 120 (22) :4383-4390

[7] Plasmacytoid monocytes migrate to inflamed lymph nodes and produce large amounts of type I interferon [J].

Cella, M ;

Jarrossay, D ;

Facchetti, F ;

Alebardi, O ;

Nakajima, H ;

Lanzavecchia, A ;

Colonna, M .

NATURE MEDICINE, 1999, 5 (08) :919-923

[8] Single-Cell RNA-Seq Technologies and Related Computational Data Analysis [J].

Chen, Geng ;

Ning, Baitang ;

Shi, Tieliu .

FRONTIERS IN GENETICS, 2019, 10

[9] Revolutionizing immunology with single-cell RNA sequencing [J].

Chen, Haide ;

Ye, Fang ;

Guo, Guoji .

CELLULAR & MOLECULAR IMMUNOLOGY, 2019, 16 (03) :242-249

[10] Performance Assessment and Selection of Normalization Procedures for Single-Cell RNA-Seq [J].

Cole, Michael B. ;

Risso, Davide ;

Wagner, Allon ;

DeTomaso, David ;

Ngai, John ;

Purdom, Elizabeth ;

Dudoit, Sandrine ;

Yosef, Nir .

CELL SYSTEMS, 2019, 8 (04) :315-+

← 1 2 3 4 5 6 7 8 →