ANALYSIS OF ANTIGEN CONSERVATION AND INACTIVATION OF GAMMA-IRRADIATED AVIAN INFLUENZA VIRUS SUBTYPE H9N2

被引：7

作者：

Salehi, Bahareh ^{[1
]}

Motamedi-Sedeh, Farahnaz ^{[2
]}

Madadgar, Omid ^{[1
]}

Khalili, Iraj ^{[3
]}

Langroudi, Arash Ghalyan Chi ^{[1
]}

Unger, Hermann ^{[4
]}

Wijewardana, Viskam ^{[4
]}

机构：

[1] Univ Tehran, Fac Vet Med, Tehran, Iran

[2] Nucl Sci & Technol Res Inst, Nucl Agr Res Sch, Tehran, Iran

[3] Razi Vaccine & Serum Res Inst, Qual Control Dept, Karaj, Iran

[4] IAEA, Anim Prod & Hlth Sect, Dept Nucl Sci & Applicat, VIC, Vienna, Austria

来源：

ACTA MICROBIOLOGICA ET IMMUNOLOGICA HUNGARICA | 2018年 / 65卷 / 02期

关键词：

avian influenza A subtype H9N2 virus; gamma irradiation; hemagglutinin antigen; inactivation; FORMALDEHYDE-INDUCED MODIFICATIONS; VACCINE; IDENTIFICATION; PROTEINS;

D O I：

10.1556/030.65.2018.025

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Avian influenza (AI) A subtype H9N2 virus belongs to Orthomyxoviridae family and causes low-pathogenic disease AI. The use of gamma-irradiated viral antigens has been developed in the production of effective vaccines. In this research, LPAIV H9N2 strain, A/Chicken/IRN/Ghazvin/2001, was multiplied on SPF eggs and irradiated by a Nordian gamma cell instrument. Irradiated and non-irradiated AI virus (AIV) samples were titrated by EID50 method and hemagglutinin (HA) antigen was analyzed by HA test as the WHO pattern method. Infectivity of irradiated virus was determined by egg inoculation method during four blind cultures. The results showed that after increasing the dose of gamma radiation, virus titer gradually decreased. D-10 value and optimum dose for complete virus inactivation were calculated by dose/response curve, 3.36 and 29.52 kGy, respectively. In addition, HA antigenicity of gamma-irradiated virus samples from 0 to 30 kGy was not changed. The results of safety test for gamma-irradiated AIV samples showed complete inactivation with gamma ray doses 30 and 35 kGy, without any multiplication on eggs after four blind cultures. According to the results of HA antigen assay and safety test, the gamma-irradiated and complete inactivated AIV subtype H9N2 is a good candidate as an inactivated immunogenic agent for poultry vaccination.

引用

页码：163 / 171

页数：9

共 22 条

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