Molecularly imprinted monolithic stationary phases for liquid chromatographic separation of enantiomers and diastereomers

被引：152

作者：

Huang, XD ^{[1
]}

Zou, HF ^{[1
]}

Chen, XM ^{[1
]}

Luo, QZ ^{[1
]}

Kong, L ^{[1
]}

机构：

[1] Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116011, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY A | 2003年 / 984卷 / 02期

基金：

中国国家自然科学基金;

关键词：

molecular imprinting; stationary phases; monolithic; LC; enantiomer separation; diastereomer separation;

D O I：

10.1016/S0021-9673(02)01768-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The method for preparation of molecularly imprinted monolithic stationary phase has been improved to achieve liquid chromatographic separation of enantiomers and diastereomers. By adopting low polar porogenic solvents of toluene and dodecanol and optimal polymerization conditions, the molecularly imprinted monolithic stationary phases with good flow-through properties and high resolution were prepared. Enantiomers of amino acid derivatives and diastereomers of cinchona alkaloids were completely resolved using the monolithic stationary phases. The influence of porogenic composition, monomer-template ratio and polymerization conditions on the chromatographic performance was investigated. Some chromatographic conditions such as the composition of the mobile phase and the temperature were characterized. Scanning electron microscopy showed that the molecularly imprinted monolithic stationary phase has a large through-pore structure to allow the mobile phase to flow through the column at very low backpressure. Accelerated separations of enantiomers and diastereomers were therefore achieved at elevated flow rates. Finally, the chiral recognition performance of the prepared stationary phase in aqueous media was investigated. Hydrophobic interaction, and ionic and/or hydrogen bonding interactions were proposed to be responsible for the recognition mechanism. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：273 / 282

页数：10

共 30 条

[1] Study of the nature of recognition in molecularly imprinted polymers, II [1] - Influence of monomer-template ratio and sample load on retention and selectivity [J].