Hamadaea flava sp nov., isolated from a soil sample and emended description of the genus Hamadaea

A Gram-stain-positive, aerobic and non-motile actinobacterial strain, designated YIM C0533(T), was isolated from a soil sample collected from Shiling county, Yunnan province, south-west China. The isolate grew at 15-37 degrees C, pH 6.0-8.0 and in the presence of 0-3 % (w/v) NaCl. The whole-cell hydrolysates contained meso-diaminopimelic acid, xylose, galactose, mannose, ribose, arabinose, glucose and rhamnose. The acyl type of muramic acid was glycolyl. The polar lipids detected were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside and an unidentified phospholipid. The major cellular fatty acids were iso-C-16 : 0, 10-methyl C-17 : 0 and iso-C-15 : 0. MK-9(H-6) was the predominant menaquinone. The genomic DNA G+ C content was determined to be 69.4 mol%. These chemotaxonomic data and the morphological properties were consistent with those of the genus Hamadaea. The strain showed highest sequence similarities to Hamadaea tsunoensis CGMCC 4.1403(T) on phylogenetic analysis of 16S rRNA gene sequences and was found to form a coherent cluster in the neighbour-joining tree. The DNA-DNA hybridization experiment indicated that the DNA-DNA relatedness value between strain YIM C0533(T) and H. tsunoensis CGMCC 4.1403(T) was 34.4 +/- 1.3 %. In addition, the results of physiological and biochemical tests allowed the isolate to be differentiated phenotypically from H. tsunoensis CGMCC 4.1403(T). On the basis of data from this polyphasic study, strain YIM C0533(T) is characterized as a novel species of the genus Hamadaea, for which the name Hamadaea flava sp. nov. is proposed. The type strain is YIM C0533(T) (=CPCC 204160(T) =KCTC 39591(T) =CGMCC 4.7289(T)). An emended description of the genus Hamadaea is also provided.