The INO80 complex is required for damage-induced recombination

被引：30
作者：
Kawashima, Satoshi
Ogiwara, Hideaki
Tada, Shusuke
Harata, Masahiko
Wintersberger, Ulrike
Enomoto, Takemi
Seki, Masayuki
机构：
[1] Tohoku Univ, Grad Sch Pharmaceut Sci, Mol Cell Biol Lab, Aoba Ku, Sendai, Miyagi 9808578, Japan
[2] Tohoku Univ, Grad Sch Agr Sci, Div Life Sci,Lab Mol Biol, Dept Mol & Cell Biol, Sendai, Miyagi 9818555, Japan
[3] Univ Vienna, Inst Canc Res, Div Mol Genet, A-1090 Vienna, Austria
[4] Tohoku Univ, 21st Century COE Program, Comprehens Res & Educ Ctr Planning Drug Dev & Cli, Sendai, Miyagi 9808578, Japan
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2007年 / 355卷 / 03期
关键词：
INO80; SWR1; NuA4; Arp4; Arp5; Arp6; Arp8; recombination; repair; chromatin remodeling;
D O I：
10.1016/j.bbrc.2007.02.036
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The budding yeast INO80 complex has a role in remodeling chromatin structure, and the SWR1 complex replaces a H2A/H2B dimer with a variant dimer, H2A.Z (Htz1)/H2B. It has been reported that these chromatin remodeling complexes contain Arp4 (actin-related protein) and actin in common and are recruited to HO endonuclease-induced DNA double-strand break (DSB) site. Reportedly, Ino80 can evict nucleosomes surrounding a HO-induced DSB; however, it has no apparent role to play in the repair of HO-induced DSB. Here we show that an essential factor for INO80 chromatin remodeling activity, Arp8, is involved in damage-induced sister chromatid recombination and interchromosomal recombination between heteroallales. In contrast, arp6 mutants are proficient for recombination, indicating that the SWRI complex does not promote recombination. Our data suggest that the remodeling of chromatin by the INO80 complex facilitates efficient homologus recombination upon DNA damages. (c) 2007 Elsevier Inc. All rights reserved.
引用
收藏
页码：835 / 841
页数：7
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