Single point mutation detection in living cancer cells by far-red emitting PNA-FIT probes

被引：47

作者：

Kolevzon, N. ^{[1
]}

Hashoul, D. ^{[1
]}

Naik, S. ^{[1
]}

Rubinstein, A. ^{[1
]}

Yavin, E. ^{[1
]}

机构：

[1] Hebrew Univ Jerusalem, Sch Pharm, Inst Drug Res, IL-91120 Jerusalem, Israel

来源：

CHEMICAL COMMUNICATIONS | 2016年 / 52卷 / 11期

基金：

以色列科学基金会;

关键词：

MESSENGER-RNA; K-RAS; COLORECTAL-CANCER; CYANINE DYES; BEACONS; DNA;

D O I：

10.1039/c5cc07502e

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Peptide nucleic acid bis-quinoline conjugates are reported as attractive far-red emitting probes that detect mutated mRNA in living cells at SNP resolution.

引用

页码：2405 / 2407

页数：3

共 24 条

[1] New cyanine dyes as base surrogates in PNA: Forced intercalation probes (FIT-probes) for homogeneous SNP detection [J].

Bethge, Lucas ;

Jarikote, Dilip Venkatrao ;

Seitz, Oliver .

BIOORGANIC & MEDICINAL CHEMISTRY, 2008, 16 (01) :114-125

[2] K-ras oncogene mutations in sporadic colorectal cancer in The Netherlands Cohort Study [J].

Brink, M ;

de Goeij, AFPM ;

Weijenberg, MP ;

Roemen, GMJM ;

Lentjes, MHFM ;

Pachen, MMM ;

Smits, KM ;

de Bruïne, AP ;

Goldbohm, RA ;

van den Brandt, PA .

CARCINOGENESIS, 2003, 24 (04) :703-710

[3] Delivery of Antisense Peptide Nucleic Acids to Cells by Conjugation with Small Arginine-Rich Cell-Penetrating Peptide (R/W)9 [J].

Cordier, Celine ;

Boutimah, Fatima ;

Bourdeloux, Mathilde ;

Dupuy, Florian ;

Met, Elisabeth ;

Alberti, Patrizia ;

Loll, Francois ;

Chassaing, Gerard ;

Burlina, Fabienne ;

Saison-Behmoaras, Tula Ester .

PLOS ONE, 2014, 9 (08)

[4] SNP Detection in mRNA in Living Cells Using Allele Specific FRET Probes [J].

Dahan, Liya ;

Huang, Lingyan ;

Kedmi, Ranit ;

Behlke, Mark A. ;

Peer, Dan .

PLOS ONE, 2013, 8 (09)

[5] STABILITY OF PEPTIDE NUCLEIC-ACIDS IN HUMAN SERUM AND CELLULAR-EXTRACTS [J].

DEMIDOV, VV ;

POTAMAN, VN ;

FRANKKAMENETSKII, MD ;

EGHOLM, M ;

BUCHARD, O ;

SONNICHSEN, SH ;

NIELSEN, PE .

BIOCHEMICAL PHARMACOLOGY, 1994, 48 (06) :1310-1313

[6] Efficient inhibition of miR-155 function in vivo by peptide nucleic acids [J].

Fabani, Martin M. ;

Abreu-Goodger, Cei ;

Williams, Donna ;

Lyons, Paul A. ;

Torres, Adrian G. ;

Smith, Kenneth G. C. ;

Enright, Anton J. ;

Gait, Michael J. ;

Vigorito, Elena .

NUCLEIC ACIDS RESEARCH, 2010, 38 (13) :4466-4475

[7] LNA-enhanced DNA FIT-probes for multicolour RNA imaging [J].

Hoevelmann, F. ;

Gaspar, I. ;

Chamiolo, J. ;

Kasper, M. ;

Steffen, J. ;

Ephrussi, A. ;

Seitz, O. .

CHEMICAL SCIENCE, 2016, 7 (01) :128-135

[8] Brightness through Local Constraint-LNA-Enhanced FIT Hybridization Probes for In Vivo Ribonucleotide Particle Tracking [J].

Hoevelmann, Felix ;

Gaspar, Imre ;

Loibl, Simon ;

Ermilov, Eugeny A. ;

Roeder, Beate ;

Wengel, Jesper ;

Ephrussi, Anne ;

Seitz, Oliver .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2014, 53 (42) :11370-11375

[9] Exploring base-pair-specific optical properties of the DNA stain thiazole orange [J].

Jarikote, Dilip Venkatrao ;

Krebs, Nils ;

Tannert, Sebastian ;

Roeder, Beate ;

Seitz, Oliver .

CHEMISTRY-A EUROPEAN JOURNAL, 2007, 13 (01) :300-310

[10] Detection of Endogenous K-ras mRNA in Living Cells at a Single Base Resolution by a PNA Molecular Beacon [J].

Kam, Yossi ;

Rubinstein, Abraham ;

Nissan, Aviram ;

Halle, David ;

Yavin, Eylon .

MOLECULAR PHARMACEUTICS, 2012, 9 (03) :685-693

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