Immunophenotypic heterogeneity of bone marrow-derived mesenchymal stromal cells from patients with hematologic disorders: correlation with bone marrow microenvironment

被引：0

作者：

Campioni, Diana ^{[1
]}

Moretti, Sabrina ^{[1
]}

Ferrari, Luisa ^{[1
]}

Punturieri, Marina ^{[1
]}

Castoldi, Gian Luigi ^{[1
]}

Lanza, Francesco ^{[1
]}

机构：

[1] Univ Hosp, Sect Hematol, Ferrara, Italy

来源：

HAEMATOLOGICA | 2006年 / 91卷 / 03期

关键词：

mesenchymal stromal cell subsets; flow cytometry; bone marrow microenvironment; hematologic disorders; angiogenesis;

D O I：

暂无

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

The immunophenotypic analysis of ex vivo-expanded mesenchymal stromal cells (MSC) has so far been confined to single or dual staining analysis in normal subjects. In this study, using a four-color cytofluorimetric protocol, we demonstrated that cultured MSC derived from the bone marrow of patients with hematologic malignancies showed alterations in the expression of CD105, CD90, CD184, and HLA-DR molecules. The decrease in the percentage of CD105(+) and CD90(+) MSC correlated with an increased bone marrow angiogenesis. This paper provides evidence that multiparametric flow cytometry is essential for the establishment of a standardized protocol to identify various MSCs subsets and aberrant phenotypes.

引用

收藏

页码：364 / 368

页数：5

相关论文

共 12 条

[1] Cotransplantation of stroma results in enhancement of engraftment and early expression of donor hematopoietic stem cells in utero [J].

Almeida-Porada, G ;

Flake, AW ;

Glimp, HA ;

Zanjani, ED .

EXPERIMENTAL HEMATOLOGY, 1999, 27 (10) :1569-1575

[2] In vitro evaluation of bone marrow angiogenesis in myelodysplastic syndromes: a morphological and functional approach [J].

Campioni, D ;

Punturieri, M ;

Bardi, A ;

Moretti, S ;

Tammiso, E ;

Lanza, F ;

Castoldi, G .

LEUKEMIA RESEARCH, 2004, 28 (01) :9-17

[3] Functional and immunophenotypic characteristics of isolated CD105+ and fibroblast+ stromal cells from AML:: implications for their plasticity along endothelial lineage [J].

Campioni, D ;

Lanza, F ;

Moretti, S ;

Dominici, M ;

Punturieri, M ;

Pauli, S ;

Hofmann, T ;

Horwitz, E ;

Castoldi, GL .

CYTOTHERAPY, 2003, 5 (01) :66-79

[4] Rapid expansion of recycling stem cells in cultures of plastic-adherent cells from human bone marrow [J].

Colter, DC ;

Class, R ;

DiGirolamo, CM ;

Prockop, DJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (07) :3213-3218

[5] Human bone marrow stromal cells suppress T-lymphocyte proliferation induced by cellular or nonspecific mitogenic stimuli [J].

Di Nicola, M ;

Carlo-Stella, C ;

Magni, M ;

Milanesi, M ;

Longoni, PD ;

Matteucci, P ;

Grisanti, S ;

Gianni, AM .

BLOOD, 2002, 99 (10) :3838-3843

[6] Hematopoietic cells and osteoblasts are derived from a common marrow progenitor after bone marrow transplantation [J].

Dominici, M ;

Pritchard, C ;

Garlits, JE ;

Hofmann, TJ ;

Persons, DA ;

Horwitz, EM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (32) :11761-11766

[7] Mesenchymal stem cells in myelodysplastic syndromes:: phenotypic and cytogenetic characterization [J].

Flores-Figueroa, E ;

Arana-Trejo, RM ;

Gutiérrez-Espíndola, G ;

Pérez-Cabrera, A ;

Mayani, H .

LEUKEMIA RESEARCH, 2005, 29 (02) :215-224

[8]

Götherström C, 2005, HAEMATOLOGICA, V90, P1017

[9] Long-term bone marrow culture-derived stromal fibroblasts as a potential target for gene therapy in acute myelogenous leukemia [J].

Min, YH ;

Li, GX ;

Jang, JH ;

Suh, HC ;

Kim, JS ;

Cheong, JW ;

Lee, ST ;

Hahn, JS ;

Ko, YW .

LEUKEMIA RESEARCH, 2002, 26 (04) :369-376

[10] Multilineage potential of adult human mesenchymal stem cells [J].

Pittenger, MF ;

Mackay, AM ;

Beck, SC ;

Jaiswal, RK ;

Douglas, R ;

Mosca, JD ;

Moorman, MA ;

Simonetti, DW ;

Craig, S ;

Marshak, DR .

SCIENCE, 1999, 284 (5411) :143-147