A minimal hybridization chain reaction (HCR) system using peptide nucleic acids

被引：36
作者：
Kim, Ki Tae [1 ]
Angerani, Simona [1 ]
Winssinger, Nicolas [1 ]
机构：
[1] Univ OfGeneva, Geneva, Switzerland
来源：
CHEMICAL SCIENCE | 2021年 / 12卷 / 23期
基金：
瑞士国家科学基金会;
关键词：
CARBONIC-ANHYDRASE-IX; STRAND-DISPLACEMENT; TEMPLATED REACTIONS; POOR-PROGNOSIS; DNA; PNA; AMPLIFICATION; RECOGNITION; MICRORNA; TARGET;
D O I：
10.1039/d1sc01269j
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
The HCR represents a powerful tool for amplification in DNA-based circuitry and sensing applications, yet requires the use of long DNA sequences to grant hairpin metastability. Here we describe a minimal HCR system based on peptide nucleic acids (PNAs). A system comprising a 5-mer stem and 5-mer loop/toehold hairpins was found to be suitable to achieve rapid amplification. These hairpins were shown to yield >10-fold amplification in 2 h and be suitable for the detection of a cancer biomarker on live cells. The use of gamma-peg-modified PNA was found to be beneficial.
引用
收藏
页码：8218 / 8223
页数：6
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