A minimal hybridization chain reaction (HCR) system using peptide nucleic acids

被引：36

作者：

Kim, Ki Tae ^{[1
]}

Angerani, Simona ^{[1
]}

Winssinger, Nicolas ^{[1
]}

机构：

[1] Univ OfGeneva, Geneva, Switzerland

来源：

CHEMICAL SCIENCE | 2021年 / 12卷 / 23期

基金：

瑞士国家科学基金会;

关键词：

CARBONIC-ANHYDRASE-IX; STRAND-DISPLACEMENT; TEMPLATED REACTIONS; POOR-PROGNOSIS; DNA; PNA; AMPLIFICATION; RECOGNITION; MICRORNA; TARGET;

D O I：

10.1039/d1sc01269j

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The HCR represents a powerful tool for amplification in DNA-based circuitry and sensing applications, yet requires the use of long DNA sequences to grant hairpin metastability. Here we describe a minimal HCR system based on peptide nucleic acids (PNAs). A system comprising a 5-mer stem and 5-mer loop/toehold hairpins was found to be suitable to achieve rapid amplification. These hairpins were shown to yield >10-fold amplification in 2 h and be suitable for the detection of a cancer biomarker on live cells. The use of gamma-peg-modified PNA was found to be beneficial.

引用

收藏

页码：8218 / 8223

页数：6

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