Chitosan Hydrogels for the Regeneration of Infarcted Myocardium: Preparation, Physicochemical Characterization, and Biological Evaluation

被引：46

作者：

Fiamingo, Anderson ^{[1
,2
]}

Montembault, Alexandra ^{[1
]}

Boitard, Solene-Emmanuelle ^{[3
]}

Naemetalla, Hany ^{[4
,5
]}

Agbulut, Onnik ^{[3
]}

Delair, Thierry ^{[1
]}

Campana-Filho, Sergio Paulo ^{[2
]}

Menasche, Philippe ^{[4
,6
]}

David, Laurent ^{[1
]}

机构：

[1] Univ Lyon 1, Ingn Mat Polymeres IMP Lyon1, CNRS, UMR 5223, 15 Bd Latarjet, F-69622 Villeurbanne, France

[2] Univ Sao Paulo, Inst Quim Sao Carlos, Ave Trabalhador Sao Carlense, BR-400 Sao Carlos, SP, Brazil

[3] Univ Paris 06, Sorbonne Univ, Biol Adaptat & Ageing, IBPS,UMR CNRS 8256, F-75005 Paris, France

[4] Univ Paris 05, Sorbonne Paris Cite, INSERM, UMR 970, F-75015 Paris, France

[5] Univ Sorbonne Paris Cite, Hop Europeen Georges Pompidou, AP HP, Dept Cardiol, F-75015 Paris, France

[6] Hop Europeen Georges Pompidou, AP HP, Dept Cardiovasc Surg, F-75015 Paris, France

来源：

BIOMACROMOLECULES | 2016年 / 17卷 / 05期

关键词：

FIBROBLAST GROWTH FACTOR-2; PHOTOCROSSLINKABLE CHITOSAN; ACETYLATED CHITOSANS; PHYSICAL HYDROGELS; AQUEOUS-SOLUTION; BETA-CHITIN; DEACETYLATION; REPAIR; MECHANISM; GELATION;

D O I：

10.1021/acs.biomac.6b00075

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The formation of chitosan hydrogels without any external cross-linking agent was successfully achieved by inducing the gelation of a viscous chitosan solution with aqueous NaOH or gaseous NH3. The hydrogels produced from high molecular weight (M-w approximate to 640 000 g mol(-1)) and extensively deacetylated chitosan (DA approximate to 2.8%) at polymer concentrations above similar to 2.0% exhibited improved mechanical properties due to the increase of the chain entanglements and intermolecular junctions. The results also show that the physicochemical and mechanical properties of chitosan hydrogels can be controlled by varying their polymer concentration and by controlling the gelation conditions, that is, by using different gelation routes. The biological evaluation of such hydrogels for regeneration of infarcted myocardium revealed that chitosan hydrogels prepared from 1.5% polymer solutions were perfectly incorporated onto the epicardial surface of the heart and presented partial degradation accompanied by mononuclear cell infiltration.

引用

页码：1662 / 1672

页数：11

共 37 条

[1] Comparison of human skeletal myoblasts and bone marrow-derived CD133+ progenitors for the repair of infarcted myocardium [J].