Fluorescence Quenching Property of C-Phycocyanin from Spirulina platensis and its Binding Efficacy with Viable Cell Components

被引：16

作者：

Paswan, Meenakshi B. ^{[1
]}

Chudasama, Meghna M. ^{[1
]}

Mitra, Madhusree ^{[2
,3
]}

Bhayani, Khushbu ^{[2
]}

George, Basil ^{[4
]}

Chatterjee, Shruti ^{[2
]}

Mishra, Sandhya ^{[2
,3
]}

机构：

[1] Gujarat Forens Sci Univ, Inst Forens Sci, DFS Campus, Gandhinagar, Gujarat, India

[2] Cent Salt & Marine Chem Res Inst, CSIR, Dept Salt & Marine Chem, Bhavnagar 364002, Gujarat, India

[3] Cent Salt & Marine Chem Res Inst, CSIR, AcSIR, Bhavnagar 364002, Gujarat, India

[4] CMS Coll, Dept Bot, Kottayam 686001, Kerala, India

来源：

JOURNAL OF FLUORESCENCE | 2016年 / 26卷 / 02期

关键词：

Microalgae; Spirulina platensis; C-phycocyanin (C-PC); Nucleic acid; Fluorescence; POTENTIAL APPLICATIONS; IMMUNOFLUORESCENCE; STABILITY;

D O I：

10.1007/s10895-015-1742-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Phycocyanin is a natural brilliant blue colored, fluorescent protein, which is commonly present in cyanobacteria. In this study, C-phycocyanin was extracted and purified from Spirulina platensis, which are multicellular and filamentous cyanobacteria of greater importance because of its various biological and pharmacological potential. It was analyzed for its binding affinity towards blood cells, algal cells, genomic DNA of microalgae, and bacteria at different temperature and incubation time. It showed good binding affinity with these components even at low concentration of 2.5 mu M. The purpose of this study was to evaluate the applicability of C-phycocyanin as a green fluorescent dye substituting carcinogenic chemical dyes.

引用

页码：577 / 583

页数：7

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