Investigation of the microbiota of the reproductive tract in women undergoing a total hysterectomy and bilateral salpingo-oopherectomy

被引:101
作者
Miles, Shana M. [1 ,2 ,4 ]
Hardy, Britney L. [3 ]
Merrell, D. Scott [3 ]
机构
[1] Walter Reed Natl Mil Med Ctr, Dept Obstet & Gynecol, Bethesda, MD USA
[2] Uniformed Serv Univ Hlth Sci, Dept Microbiol & Immunol, Bethesda, MD USA
[3] Uniformed Serv Univ Hlth Sci, Bethesda, MD USA
[4] Ehrling Bergquist Womens Hlth Clin, 2501 Capehart Rd, Offutt AFB, NE 68113 USA
关键词
Reproductive tract microbiota; upper genital tract colonization; ovarian microbiota; salpingitis; fallopian tube microbiota; VAGINAL MICROBIOME; HYDROSALPINX FLUID; COMMUNITIES; COLONIZATION; ASSOCIATION; DYNAMICS; DISEASES; IMPACT;
D O I
10.1016/j.fertnstert.2016.11.028
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
Objective: To conduct a pilot study to investigate the possible presence of bacteria throughout the female reproductive tract and to make a preliminary assessment of whether there are differences in the composition of the microbial communities between these body sites and/or between patients. Design: Prospective pilot study followed by 16S amplification and high-throughput sequencing. Setting: Tertiary care military hospital. Patient(s): A total of 10 women underwent a total hysterectomy with bilateral salpingo-oopherectomy; tissue samples were collected from the vagina, resected cervix, uterus, fallopian tubes, and ovaries. Intervention(s): None. Main Outcome Measure(s): Microbial composition of samples within patients and between body sites. Result(s): The microbial composition of each sample was characterized by amplification and sequencing of the V1-V3 region of the 16S rRNA gene. Bacteria were identified in 95% of the samples; the remaining 5% of samples showed no evidence of bacterial 16S rRNA. The microbial communities present at each anatomical location were highly related across the samples and across the patients. The Firmicutes phylum was highly abundant as was the Lactobacillus genus. Conclusion(s): This study is the first global evaluation of the distribution of bacteria throughout the female reproductive tract in its entirety. Bacteria were detected by 16S sequencing from anatomical sites including the fallopian tubes and ovaries. The microbial profiles were closely related regardless of which body site or patient the samples originated from. The results of this trial will serve as the basis for future work correlating the colonization of the female reproductive tract with both obstetric and gynecologic conditions. (C) 2016 by American Society for Reproductive Medicine.
引用
收藏
页码:813 / +
页数:9
相关论文
共 34 条
[1]   A Metagenomic Approach to Characterization of the Vaginal Microbiome Signature in Pregnancy [J].
Aagaard, Kjersti ;
Riehle, Kevin ;
Ma, Jun ;
Segata, Nicola ;
Mistretta, Toni-Ann ;
Coarfa, Cristian ;
Raza, Sabeen ;
Rosenbaum, Sean ;
Van den Veyver, Ignatia ;
Milosavljevic, Aleksandar ;
Gevers, Dirk ;
Huttenhower, Curtis ;
Petrosino, Joseph ;
Versalovic, James .
PLOS ONE, 2012, 7 (06)
[2]   Association of asymptomatic bacterial vaginosis with endometrial microbial colonization and plasma cell endometritis in nonpregnant women [J].
Andrews, William W. ;
Hauth, John C. ;
Cliver, Suzanne P. ;
Conner, Michael G. ;
Goldenberg, Robert L. ;
Goepfert, Alice R. .
AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY, 2006, 195 (06) :1611-1616
[3]   Interplay Between the Temporal Dynamics of the Vaginal Microbiota and Human Papillomavirus Detection [J].
Brotman, Rebecca M. ;
Shardell, Michelle D. ;
Gajer, Pawel ;
Tracy, J. Kathleen ;
Zenilman, Jonathan M. ;
Ravel, Jacques ;
Gravitt, Patti E. .
JOURNAL OF INFECTIOUS DISEASES, 2014, 210 (11) :1723-1733
[4]   Association between the vaginal microbiota, menopause status, and signs of vulvovaginal atrophy [J].
Brotman, Rebecca M. ;
Shardell, Michelle D. ;
Gajer, Pawel ;
Fadrosh, Doug ;
Chang, Kathryn ;
Silver, Michelle I. ;
Viscidi, Raphael P. ;
Burke, Anne E. ;
Ravel, Jacques ;
Gravitt, Patti E. .
MENOPAUSE-THE JOURNAL OF THE NORTH AMERICAN MENOPAUSE SOCIETY, 2014, 21 (05) :450-458
[5]   Gene, environment, microbiome and mucosal immune tolerance in rheumatoid arthritis [J].
Catrina, Anca I. ;
Deane, Kevin D. ;
Scher, Jose U. .
RHEUMATOLOGY, 2016, 55 (03) :391-402
[6]   Temporal and spatial variation of the human microbiota during pregnancy [J].
DiGiulio, Daniel B. ;
Callahan, Benjamin J. ;
McMurdie, Paul J. ;
Costello, Elizabeth K. ;
Lyell, Deirdre J. ;
Robaczewska, Anna ;
Sun, Christine L. ;
Goltsman, Daniela S. A. ;
Wong, Ronald J. ;
Shaw, Gary ;
Stevenson, David K. ;
Holmes, Susan P. ;
Relman, David A. .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2015, 112 (35) :11060-11065
[7]   Species-level classification of the vaginal microbiome [J].
Fettweis, Jennifer M. ;
Serrano, Myrna G. ;
Sheth, Nihar U. ;
Mayer, Carly M. ;
Glascock, Abigail L. ;
Brooks, J. Paul ;
Jefferson, Kimberly K. ;
Buck, Gregory A. .
BMC GENOMICS, 2012, 13
[8]   Ovarian Cancer: The Fallopian Tube as the Site of Origin and Opportunities for Prevention [J].
George, Sophia H. L. ;
Garcia, Ruslan ;
Slomovitz, Brian M. .
FRONTIERS IN ONCOLOGY, 2016, 6
[9]   Effects of tampons and menses on the composition and diversity of vaginal microbial communities over time [J].
Hickey, R. J. ;
Abdo, Z. ;
Zhou, X. ;
Nemeth, K. ;
Hansmann, M. ;
Osborn, T. W., III ;
Wang, F. ;
Forney, L. J. .
BJOG-AN INTERNATIONAL JOURNAL OF OBSTETRICS AND GYNAECOLOGY, 2013, 120 (06) :695-706
[10]   Vaginal Microbiota of Adolescent Girls Prior to the Onset of Menarche Resemble Those of Reproductive-Age Women [J].
Hickey, Roxana J. ;
Zhou, Xia ;
Settles, Matthew L. ;
Erb, Julie ;
Malone, Kristin ;
Hansmann, Melanie A. ;
Shew, Marcia L. ;
Van der Pol, Barbara ;
Fortenberry, J. Dennis ;
Forney, Larry J. .
MBIO, 2015, 6 (02)