The Complete Sequence of a Human Parainfluenzavirus 4 Genome

被引：10

作者：

Yea, Carmen ^{[3
]}

Cheung, Rose ^{[1
]}

Collins, Carol ^{[1
]}

Adachi, Dena ^{[1
]}

Nishikawa, John ^{[1
]}

Tellier, Raymond ^{[1
,2
,3
]}

机构：

[1] Hosp Sick Children, Div Microbiol, Toronto, ON M5G 1X8, Canada

[2] Univ Toronto, Dept Lab Med & Pathobiol, Toronto, ON, Canada

[3] Hosp Sick Children, Res Inst, Program Genet & Genome Biol, Toronto, ON M5G 1X8, Canada

来源：

VIRUSES-BASEL | 2009年 / 1卷 / 01期

关键词：

human parainfluenza virus 4; complete genome sequence; L protein; long RT-PCR; MOLECULAR-CLONING; VIRUS TYPE-4A; GENE; PARAMYXOVIRUS; REPLICATION; RULE;

D O I：

10.3390/v1010026

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Although the human parainfluenza virus 4 (HPIV4) has been known for a long time, its genome, alone among the human paramyxoviruses, has not been completely sequenced to date. In this study we obtained the first complete genomic sequence of HPIV4 from a clinical isolate named SKPIV4 obtained at the Hospital for Sick Children in Toronto (Ontario, Canada). The coding regions for the N, P/V, M, F and HN proteins show very high identities (95% to 97%) with previously available partial sequences for HPIV4B. The sequence for the L protein and the non-coding regions represent new information. A surprising feature of the genome is its length, more than 17 kb, making it the longest genome within the genus Rubulavirus, although the length is well within the known range of 15 kb to 19 kb for the subfamily Paramyxovirinae. The availability of a complete genomic sequence will facilitate investigations on a respiratory virus that is still not completely characterized.

引用

页码：26 / 41

页数：16

共 30 条

[1] Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].

Altschul, SF ;

Madden, TL ;

Schaffer, AA ;

Zhang, JH ;

Zhang, Z ;

Miller, W ;

Lipman, DJ .

NUCLEIC ACIDS RESEARCH, 1997, 25 (17) :3389-3402

[2]

[Anonymous], CLIN VIROLOGY MANUAL

[3] MOLECULAR-CLONING AND SEQUENCE-ANALYSIS OF HUMAN PARAINFLUENZA TYPE-4A VIRUS HN GENE - ITS IRREGULARITIES ON STRUCTURE AND ACTIVITIES [J].

BANDO, H ;

KONDO, K ;

KAWANO, M ;

KOMADA, H ;

TSURUDOME, M ;

NISHIO, M ;

ITO, Y .

VIROLOGY, 1990, 175 (01) :307-312

[4]

Chanock R.M., 2001, Fields virology, V4th, P1341

[5] The complete sequence of the bovine torovirus genome [J].

Draker, R ;

Roper, RL ;

Petric, M ;

Tellier, R .

VIRUS RESEARCH, 2006, 115 (01) :56-68

[6] Minimum protein requirements for transcription and RNA replication of a minigenome of human parainfluenza virus type 3 and evaluation of the rule of six [J].

Durbin, AP ;

Siew, JW ;

Murphy, BR ;

Collins, PL .

VIROLOGY, 1997, 234 (01) :74-83

[7] How Escherichia coli can bias the results of molecular cloning: Preferential selection of defective genomes of hepatitis C virus during the cloning procedure [J].

Forns, X ;

Bukh, J ;

Purcell, RH ;

Emerson, SU .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (25) :13909-13914

[8] Comparison of a commercial qualitative real-time RT-PCR kit with direct immunofluorescence assay (DFA) and cell culture for detection of influenza A and B in children [J].

Gharabaghi, Farhad ;

Tellier, Raymond ;

Cheung, Rose ;

Collins, Carol ;

Broukhanski, George ;

Drews, Steven J. ;

Richardson, Susan E. .

JOURNAL OF CLINICAL VIROLOGY, 2008, 42 (02) :190-193

[9] Genetic characterization of Nipah virus, Bangladesh, 2004 [J].

Harcourt, BH ;

Lowe, L ;

Tamin, A ;

Liu, X ;

Bankamp, B ;

Bowden, N ;

Rollin, PE ;

Comer, JA ;

Ksiazek, TG ;

Hossain, MJ ;

Gurley, ES ;

Breiman, RF ;

Bellini, WJ ;

Rota, PA .

EMERGING INFECTIOUS DISEASES, 2005, 11 (10) :1594-1597

[10] The complete genome sequence of J virus reveals a unique genome structure in the family Paramyxoviridae [J].

Jack, PJM ;

Boyle, DB ;

Eaton, BT ;

Wang, LF .

JOURNAL OF VIROLOGY, 2005, 79 (16) :10690-10700

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