Sal k 4, a New Allergen of Salsola kali, Is Profilin: A Predictive Value of Conserved Conformational Regions in Cross-Reactivity with Other Plant-Derived Profilins

被引：24

作者：

Assarehzadegan, Mohammad Ali ^{[2
]}

Amini, Akram ^{[3
]}

Sankian, Mojtaba ^{[1
]}

Tehrani, Mohsen ^{[3
]}

Jabbari, Farahzad ^{[1
]}

Varasteh, AbdolReza ^{[1
]}

机构：

[1] Mashhad Univ Med Sci, Avicenna Res Inst, Immunol Res Ctr, Mashhad, Iran

[2] Ahvaz Jundishapur Univ Med Sci, Fac Med, Dept Immunol, Ahvaz, Iran

[3] Avicenna Res Inst, Immunol Res Ctr, Immunobiochem Lab, Mashhad, Iran

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2010年 / 74卷 / 07期

关键词：

Sal k 4; Salsola kali; profilin; cross-reactivity; POLLEN; SENSITIZATION; EXPRESSION; CLONING; FAMILY;

D O I：

10.1271/bbb.100129

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The aim of this study was to investigate a new allergen of Salsola kali, Sal k 4, and to investigate the predictive value of the conserved conformational regions in cross-reactivity with other plant-derived profilins. The Sal k 4-coding sequence was cloned, expressed, and purified by one-step Ni2+ affinity chromatography to recover high-purity target protein. We assessed cross-reactivity and predicted conserved conformational regions among rSal k 4 and other plant-derived profilins. Immunodetection and inhibition assays using 30 individual sera from S. kali allergic patients indicated that purified rSal k 4 might be the same as that in the crude extract. The results of inhibition assays among rSal k 4 and other plant-derived profilins were in accordance with the homology of the predicted conserved conformational regions. Amino acid sequence homology analysis showed that a high degree of IgE cross-reactivity among plant-derived profilins might depend on the predicted conserved conformational regions.

引用

页码：1441 / 1446

页数：6

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