The CSF in neurosarcoidosis contains consistent clonal expansion of CD8 T cells, but not CD4 T cells

被引：15

作者：

Paley, Michael A. ^{[1
]}

Baker, Brandi J. ^{[2
]}

Dunham, S. Richard ^{[2
]}

Linskey, Nicole ^{[1
]}

Cantoni, Claudia ^{[2
]}

Lee, Kenneth ^{[2
]}

Hassman, Lynn M. ^{[3
]}

Laurent, Jennifer ^{[1
]}

Roberson, Elisha D. O. ^{[1
,4
]}

Clifford, David B. ^{[2
]}

Yokoyama, Wayne M. ^{[1
]}

机构：

[1] Washington Univ, Dept Med, Sch Med, St Louis, MO 63110 USA

[2] Washington Univ, Dept Neurol, Sch Med, St Louis, MO 63110 USA

[3] Washington Univ, Dept Ophthalmol & Visual Sci, Sch Med, St Louis, MO 63110 USA

[4] Washington Univ, Dept Genet, Sch Med, St Louis, MO 63110 USA

来源：

JOURNAL OF NEUROIMMUNOLOGY | 2022年 / 367卷

基金：

新加坡国家研究基金会;

关键词：

Neurosarcoidosis; Sarcoidosis; T cells; TCR sequencing; Single-cell RNA sequencing; Interferon; PULMONARY SARCOIDOSIS; GENE USAGE; EBI2; MEMORY; LYMPHOCYTES; TOFACITINIB; MIGRATION; BLOOD; FLUID; FATE;

D O I：

10.1016/j.jneuroim.2022.577860

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The tissue-specific drivers of neurosarcoidosis remain poorly defined. To identify cerebrospinal fluid (CSF) specific, antigen-driven T and B cell responses, we performed single-cell RNA sequencing of CSF and blood cells from neurosarcoid participants coupled to T and B cell receptor sequencing. In contrast to pulmonary sarcoidosis, which is driven by CD4 T cells, we found CD8 T cell clonal expansion enriched in the neurosarcoid CSF. These CSF-enriched CD8 T cells were composed of two subsets with differential expression of EBI2, CXCR3, and CXCR4. Lastly, our data suggest that IFN gamma signaling may distinguish neurosarcoidosis from other neurological disorders.

引用

页数：11

共 58 条

[21] Lofgren's Syndrome Human Leukocyte Antigen Strongly Influences the Disease Course [J].

Grunewald, Johan ;

Eklund, Anders .

AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 2009, 179 (04) :307-312

[22] Normalization and variance stabilization of single-cell RNA-seq data using regularized negative binomial regression [J].

Hafemeister, Christoph ;

Satija, Rahul .

GENOME BIOLOGY, 2019, 20 (01)

[23]

Handcock, 2006, RELATIVE DISTRIBUTIO

[24] Distinct Profiles of Cytotoxic Granules in Memory CD8 T Cells Correlate with Function, Differentiation Stage, and Antigen Exposure [J].

Harari, Alexandre ;

Enders, Felicitas Bellutti ;

Cellerai, Cristina ;

Bart, Pierre-Alexandre ;

Pantaleo, Giuseppe .

JOURNAL OF VIROLOGY, 2009, 83 (07) :2862-2871

[25]

Hassman L.M., 2021, OPHTHALMOL SCI, V1

[26] Leukocyte profiles in blood and CSF distinguish neurosarcoidosis from multiple sclerosis [J].

Heming, Michael ;

Lohmann, Lisa ;

Schulte-Mecklenbeck, Andreas ;

Brix, Tobias ;

Gross, Catharina C. ;

Wiendl, Heinz ;

Klotz, Luisa ;

zu Hoerste, Gerd Meyer .

JOURNAL OF NEUROIMMUNOLOGY, 2020, 341

[27] Human CCR5high effector memory cells perform CNS parenchymal immune surveillance via GZMK-mediated transendothelial diapedesis [J].

Herich, Sebastian ;

Schneider-Hohendorf, Tilman ;

Rohlmann, Astrid ;

Ghadiri, Maryam Khaleghi ;

Schulte-Mecklenbeck, Andreas ;

Zondler, Lisa ;

Janoschka, Claudia ;

Ostkamp, Patrick ;

Richter, Jannis ;

Breuer, Johanna ;

Dimitrov, Stoyan ;

Rammensee, Hans-Georg ;

Grauer, Oliver M. ;

Klotz, Luisa ;

Gross, Catharina C. ;

Stummer, Walter ;

Missler, Markus ;

Zarbock, Alexander ;

Vestweber, Dietmar ;

Wiendl, Heinz ;

Schwab, Nicholas .

BRAIN, 2019, 142 :3411-3427

[28] Tofacitinib for cutaneous and pulmonary sarcoidosis: A case series [J].

Kerkemeyer, Karolina L. ;

Meah, Nekma ;

Sinclair, Rodney D. .

JOURNAL OF THE AMERICAN ACADEMY OF DERMATOLOGY, 2021, 84 (02) :581-583

[29] Sarcoidosis of the central nervous system: clinical features, imaging, and CSF results [J].

Kidd, Desmond P. .

JOURNAL OF NEUROLOGY, 2018, 265 (08) :1906-1915

[30] EBI2 augments Tfh cell fate by promoting interaction with IL-2-quenching dendritic cells [J].

Li, Jianhua ;

Lu, Erick ;

Yi, Tangsheng ;

Cyster, Jason G. .

NATURE, 2016, 533 (7601) :110-+

← 1 2 3 4 5 6 →