The CSF in neurosarcoidosis contains consistent clonal expansion of CD8 T cells, but not CD4 T cells

被引：15

作者：

Paley, Michael A. ^{[1
]}

Baker, Brandi J. ^{[2
]}

Dunham, S. Richard ^{[2
]}

Linskey, Nicole ^{[1
]}

Cantoni, Claudia ^{[2
]}

Lee, Kenneth ^{[2
]}

Hassman, Lynn M. ^{[3
]}

Laurent, Jennifer ^{[1
]}

Roberson, Elisha D. O. ^{[1
,4
]}

Clifford, David B. ^{[2
]}

Yokoyama, Wayne M. ^{[1
]}

机构：

[1] Washington Univ, Dept Med, Sch Med, St Louis, MO 63110 USA

[2] Washington Univ, Dept Neurol, Sch Med, St Louis, MO 63110 USA

[3] Washington Univ, Dept Ophthalmol & Visual Sci, Sch Med, St Louis, MO 63110 USA

[4] Washington Univ, Dept Genet, Sch Med, St Louis, MO 63110 USA

来源：

JOURNAL OF NEUROIMMUNOLOGY | 2022年 / 367卷

基金：

新加坡国家研究基金会;

关键词：

Neurosarcoidosis; Sarcoidosis; T cells; TCR sequencing; Single-cell RNA sequencing; Interferon; PULMONARY SARCOIDOSIS; GENE USAGE; EBI2; MEMORY; LYMPHOCYTES; TOFACITINIB; MIGRATION; BLOOD; FLUID; FATE;

D O I：

10.1016/j.jneuroim.2022.577860

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The tissue-specific drivers of neurosarcoidosis remain poorly defined. To identify cerebrospinal fluid (CSF) specific, antigen-driven T and B cell responses, we performed single-cell RNA sequencing of CSF and blood cells from neurosarcoid participants coupled to T and B cell receptor sequencing. In contrast to pulmonary sarcoidosis, which is driven by CD4 T cells, we found CD8 T cell clonal expansion enriched in the neurosarcoid CSF. These CSF-enriched CD8 T cells were composed of two subsets with differential expression of EBI2, CXCR3, and CXCR4. Lastly, our data suggest that IFN gamma signaling may distinguish neurosarcoidosis from other neurological disorders.

引用

页数：11

共 58 条

[1]

Alam M., JAAD CASE REP, V9, P1

[2] Distinguishing neurosarcoidosis from multiple sclerosis based on CSF analysis A retrospective study [J].

Arun, Tarunya ;

Pattison, Laura ;

Palace, Jacqueline .

NEUROLOGY, 2020, 94 (24) :E2545-E2554

[3] VDJdb in 2019: database extension, new analysis infrastructure and a T-cell receptor motif compendium [J].

Bagaev, Dmitry, V ;

Vroomans, Renske M. A. ;

Samir, Jerome ;

Stervbo, Ulrik ;

Rius, Cristina ;

Dolton, Garry ;

Greenshields-Watson, Alexander ;

Attaf, Meriem ;

Egorov, Evgeny S. ;

Zvyagin, Ivan, V ;

Babel, Nina ;

Cole, David K. ;

Godkin, Andrew J. ;

Sewell, Andrew K. ;

Kesmir, Can ;

Chudakov, Dmitriy M. ;

Luciani, Fabio ;

Shugay, Mikhail .

NUCLEIC ACIDS RESEARCH, 2020, 48 (D1) :D1057-D1062

[4] The Chemoattractant Receptor Ebi2 Drives Intranodal Naive CD4+ T Cell Peripheralization to Promote Effective Adaptive Immunity [J].

Baptista, Antonio P. ;

Gola, Anita ;

Huang, Yuefeng ;

Milanez-Almeida, Pedro ;

Torabi-Parizi, Parizad ;

Urban, Joseph F., Jr. ;

Shapiro, Virginia S. ;

Gerner, Michael Y. ;

Germain, Ronald N. .

IMMUNITY, 2019, 50 (05) :1188-+

[5] Retrospective, dual-centre review of imaging findings in neurosarcoidosis at presentation: prevalence and imaging sub-types [J].

Bathla, G. ;

Freeman, C. W. ;

Moritani, T. ;

Song, J. W. ;

Srivastava, S. ;

Soni, N. ;

Derdeyn, C. ;

Mohan, S. .

CLINICAL RADIOLOGY, 2020, 75 (10) :796.e1-796.e9

[6] Mapping the immune environment in clear cell renal carcinoma by single-cell genomics [J].

Borcherding, Nicholas ;

Vishwakarma, Ajaykumar ;

Voigt, Andrew P. ;

Bellizzi, Andrew ;

Kaplan, Jacob ;

Nepple, Kenneth ;

Salem, Aliasger K. ;

Jenkins, Russell W. ;

Zakharia, Yousef ;

Zhang, Weizhou .

COMMUNICATIONS BIOLOGY, 2021, 4 (01)

[7] Neurosarcoidosis Pathophysiology, Diagnosis, and Treatment [J].

Bradshaw, Michael J. ;

Pawate, Siddharama ;

Koth, Laura L. ;

Cho, Tracey A. ;

Gelfand, Jeffrey M. .

NEUROLOGY-NEUROIMMUNOLOGY & NEUROINFLAMMATION, 2021, 8 (06)

[8] CXCR3 ligands are augmented during the pathogenesis of pulmonary sarcoidosis [J].

Busuttil, A. ;

Weigt, S. S. ;

Keane, M. P. ;

Xue, Y. Y. ;

Palchevskiy, V. ;

Burdick, M. D. ;

Huang, C. ;

Zisman, D. A. ;

Fishbein, M. ;

Lynch, J. P., III ;

Strieter, R. M. ;

Elashoff, R. M. ;

Belperio, J. A. .

EUROPEAN RESPIRATORY JOURNAL, 2009, 34 (03) :676-686

[9] MHC-I expression renders catecholaminergic neurons susceptible to T-cell-mediated degeneration [J].

Cebrian, Carolina ;

Zucca, Fabio A. ;

Mauri, Pierluigi ;

Steinbeck, Julius A. ;

Studer, Lorenz ;

Scherzer, Clemens R. ;

Kanter, Ellen ;

Budhu, Sadna ;

Mandelbaum, Jonathan ;

Vonsattel, Jean P. ;

Zecca, Luigi ;

Loike, John D. ;

Sulzer, David .

NATURE COMMUNICATIONS, 2014, 5

[10] EBI2 Expression and Function: Robust in Memory Lymphocytes and Increased by Natalizumab in Multiple Sclerosis [J].

Clottu, Aurelie S. ;

Mathias, Amandine ;

Sailer, Andreas W. ;

Schluep, Myriam ;

Seebach, Jorg D. ;

Du Pasquier, Renaud ;

Pot, Caroline .

CELL REPORTS, 2017, 18 (01) :213-224

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