U2 small nuclear ribonucleoprotein particle (snRNP) auxiliary factor of 65 kDa, U2AF65, can promote U1 snRNP recruitment to 5′ splice sites

被引：16
作者：
Förch, P [1 ]
Merendino, L [1 ]
Martínez, C [1 ]
Valcárcel, J [1 ]
机构：
[1] European Mol Biol Lab, Gene Express Programme, D-69117 Heidelberg, Germany
来源：
BIOCHEMICAL JOURNAL | 2003年 / 372卷 / 01期
关键词：
arginine- and serine-rich domain (RS domain); psoralen; sex-lethal; splicing; U1 small nuclear ribonucleoparticle (snRNP); U2 auxiliary factor;
D O I：
10.1042/BJ20021202
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The splicing factor U2AF(65) , U2 small nuclear ribonucleoprotein particle (snRNP) auxiliary factor of 65 kDa, binds to pyrimidine-rich sequences at 3' splice sites to recruit U2 snRNP to premRNAs. We report that U2AF 15 can also promote the recruitment of U I snRNP to weak 5' splice sites that are followed by uridine-rich sequences. The arginine- and serine-rich domain of U2AF(65) is critical for U I recruitment, and we discuss the role of its RNA-RNA annealing activity in this novel function of U2AF(65).
引用
收藏
页码：235 / 240
页数：6
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