ELIMINATING FALSE-POSITIVE RESULTS IN SERUM TESTS FOR NEUROMUSCULAR AUTOIMMUNITY

被引：15

作者：

Apiwattanakul, Metha ^{[1
]}

McKeon, Andrew ^{[1
,2
]}

Pittock, Sean J. ^{[1
,2
]}

Kryzer, Thomas J. ^{[1
]}

Lennon, Vanda A. ^{[1
,2
,3
]}

机构：

[1] Mayo Clin, Coll Med, Dept Lab Med & Pathol, Rochester, MN 55905 USA

[2] Mayo Clin, Coll Med, Dept Neurol, Rochester, MN 55905 USA

[3] Mayo Clin, Coll Med, Dept Immunol, Rochester, MN 55905 USA

来源：

MUSCLE & NERVE | 2010年 / 41卷 / 05期

关键词：

acetylcholine receptor autoantibody; alpha-bungarotoxin; myasthenia gravis; potassium channel autoantibody; alpha-dendrotoxin; MYASTHENIA-GRAVIS; SEROPOSITIVE MG; ANTIBODIES; CHANNEL; AB;

D O I：

10.1002/mus.21653

中图分类号：

R74 [神经病学与精神病学];

学科分类号：

摘要：

In this study we describe the false-positive frequency in radioimmunoprecipitation assays for muscle acetylcholine receptor (AChR) and neuronal voltage-gated potassium channel (VGKC) autoantibodies, attributable to I-125-ligand immunoprecipitation. Sera were evaluated for AChR autoantibody (n = 34,095) and VGKC autoantibody (n = 11,028). We retested sera that yielded apparently positive results with I-125-ligand with and without detergent-solubilized cation-channel protein and indentified clinically validated fals-positive rates of 0.05% and 1.7% for AchR and VGKC autoantibodies, respectively. Specificity assurance in radioimmunoprecipitation assays requires subtraction of values for I-125-ligand binding. Muscle Nerve 41: 702-704, 2010

引用

页码：702 / 704

页数：3

共 11 条

[1] AQP4-IgG Immunoprecipitation Assay Optimization [J].

Apiwattanakul, Metha ;

McKeon, Andrew ;

Fryer, James P. ;

Hinson, Shannon R. ;

Pittock, Sean J. ;

Lennon, Vanda A. .

CLINICAL CHEMISTRY, 2009, 55 (03) :592-594

[2]

Griesmann GE., 2002, Manual of Clinical Laboratory Immunology, V6th ed, P1005

[3]

Howard F M., 1987, Ann N Y Acad Sci, V505, P526

[4] EVIDENCE AGAINST ACETYLCHOLINE-RECEPTOR HAVING A MAIN IMMUNOGENIC REGION AS TARGET FOR AUTOANTIBODIES IN MYASTHENIA-GRAVIS [J].

LENNON, VA ;

GRIESMANN, GE .

NEUROLOGY, 1989, 39 (08) :1069-1076

[5]

Lennon Vanda A., 1997, Neurology, V48, pS23

[6] MuSK Ab described in seropositive MG sera found to be Ab to alkaline phosphatase [J].

Ohta, K ;

Shigemoto, K ;

Kubo, S ;

Maruyama, N ;

Abe, Y ;

Ueda, N ;

Fujinami, A ;

Ohta, M .

NEUROLOGY, 2005, 65 (12) :1988-1988

[7] MuSK antibodies in AChR Ab-seropositive MG vs AChR Ab-seronegative MG [J].

Ohta, K ;

Shigemoto, K ;

Kubo, S ;

Maruyama, N ;

Abe, Y ;

Ueda, N ;

Ohta, M .

NEUROLOGY, 2004, 62 (11) :2132-2133

[8] Clinical spectrum of voltage-gated potassium channel autoimmunity [J].

Tan, K. M. ;

Lennon, V. A. ;

Klein, C. J. ;

Boeve, B. F. ;

Pittock, S. J. .

NEUROLOGY, 2008, 70 (20) :1883-1890

[9] Ion channel and striational antibodies define a continuum of autoimmune neuromuscular hyperexcitability [J].

Vernino, S ;

Lennon, VA .

MUSCLE & NERVE, 2002, 26 (05) :702-707

[10]

VERNINO S, 2002, MANUAL CLIN LAB IMMU, P1013

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