Effects of light-emitting diodes (LEDs) on the accumulation of lipid content using a two-phase culture process with three microalgae

被引:91
作者
Ra, Chae-Hun [1 ]
Kang, Chang-Han [2 ]
Jung, Jang-Hyun [1 ]
Jeong, Gwi-Taek [1 ]
Kim, Sung-Koo [1 ]
机构
[1] Pukyong Natl Univ, Dept Biotechnol, Busan 48513, South Korea
[2] Busan Technopk Marine Bioind Dev Ctr MBDC, Busan 46048, South Korea
关键词
Microalgae; Lipid content; Fatty acid; Light-emitting diodes (LEDs); Two-phase culture; FATTY-ACID-COMPOSITION; CHLORELLA-VULGARIS; MARINE MICROALGAE; GROWTH; TEMPERATURE; INTENSITY; QUALITY; NITRATE;
D O I
10.1016/j.biortech.2016.04.059
中图分类号
S2 [农业工程];
学科分类号
0828 ;
摘要
Conditions of light-emitting diode (LED) wavelength, light intensity, nitrate concentration, and time of exposure to green LED light stress in a two-phase culture were optimized for lipid production with three species of microalgae. Among the three microalgae, Nannochloropsis oculata showed the highest specific growth rate (mu(max)) of 0.037 h (1) and the lowest saturation constant (K-s) of 1.32 mg/L. The highest lipid contents of the three microalgae in the second phase under green LED light stress were 52.0% (w/w) for Nannochloropsis sauna at 2 days, 53.0% (w/w) for Nannochloropsis oceanica at 2 days, and 56.0% for N. oculata at 2 days. Fatty acid analysis of the microalgae showed that 85-87% (w/w) of total fatty acids from N. satina, N. oceanica, and N. oculata consisted of palmitic acid (C16:0) and oleic acid (C18:1). (C) 2016 Published by Elsevier Ltd.
引用
收藏
页码:254 / 261
页数:8
相关论文
共 27 条
[1]  
BLIGH EG, 1959, CAN J BIOCHEM PHYS, V37, P911
[2]   Light requirements in microalgal photobioreactors: an overview of biophotonic aspects [J].
Carvalho, Ana P. ;
Silva, Susana O. ;
Baptista, Jose M. ;
Malcata, F. Xavier .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2011, 89 (05) :1275-1288
[3]   Enhanced growth and lipid production of microalgae under mixotrophic culture condition: Effect of light intensity, glucose concentration and fed-batch cultivation [J].
Cheirsilp, Benjamas ;
Torpee, Salwa .
BIORESOURCE TECHNOLOGY, 2012, 110 :510-516
[4]   Engineering strategies for enhancing the production of eicosapentaenoic acid (EPA) from an isolated microalga Nannochloropsis oceanica CY2 [J].
Chen, Chun-Yen ;
Chen, Yu-Chun ;
Huang, Hsiao-Chen ;
Huang, Chieh-Chen ;
Lee, Wen-Lung ;
Chang, Jo-Shu .
BIORESOURCE TECHNOLOGY, 2013, 147 :160-167
[5]   An optical method for the rapid measurement of micromolar concentrations of nitrate in marine phytoplankton cultures [J].
Collos, Y ;
Mornet, F ;
Sciandra, A ;
Waser, N ;
Larson, A ;
Harrison, PJ .
JOURNAL OF APPLIED PHYCOLOGY, 1999, 11 (02) :179-184
[6]   Effect of temperature and nitrogen concentration on the growth and lipid content of Nannochloropsis oculata and Chlorella vulgaris for biodiesel production [J].
Converti, Attilio ;
Casazza, Alessandro A. ;
Ortiz, Erika Y. ;
Perego, Patrizia ;
Del Borghi, Marco .
CHEMICAL ENGINEERING AND PROCESSING-PROCESS INTENSIFICATION, 2009, 48 (06) :1146-1151
[7]   Enhanced algae growth in both phototrophic and mixotrophic culture under blue light [J].
Das, Probir ;
Lei, Wang ;
Aziz, Siti Sarah ;
Obbard, Jeffrey Philip .
BIORESOURCE TECHNOLOGY, 2011, 102 (04) :3883-3887
[8]  
Fu W., 2012, APPL MICROBIOL BIOT, V12, P4502
[9]   STUDIES OF MARINE PLANKTONIC DIATOMS .1. CYCLOTELLA NANA HUSTEDT, AND DETONULA CONFERVACEA (CLEVE) GRAN [J].
GUILLARD, RR ;
RYTHER, JH .
CANADIAN JOURNAL OF MICROBIOLOGY, 1962, 8 (02) :229-&
[10]   Growth, Lipid Content, Productivity, and Fatty Acid Composition of Tropical Microalgae for Scale-Up Production [J].
Huerlimann, Roger ;
de Nys, Rocky ;
Heimann, Kirsten .
BIOTECHNOLOGY AND BIOENGINEERING, 2010, 107 (02) :245-257