Enhancement of biosensing performance in a droplet-based bioreactor by in situ microstreaming

被引：11

作者：

Ducloux, Olivier ^{[1
]}

Galopin, Elisabeth ^{[1
]}

Zoueshtiagh, Farzam ^{[1
,2
]}

Merlen, Alain ^{[1
,2
]}

Thomy, Vincent ^{[1
]}

机构：

[1] Inst Elect Microelect & Nanotechnol, F-59650 Villeneuve Dascq, France

[2] Lab Europeen Associe Magnetoacoust Non Lineaire, F-59650 Villeneuve Dascq, France

来源：

BIOMICROFLUIDICS | 2010年 / 4卷 / 01期

关键词：

acoustic streaming; bioMEMS; biosensors; chemical sensors; drops; finite element analysis; flow visualisation; microfluidics; microsensors; surface acoustic wave sensors; surface acoustic wave transducers;

D O I：

10.1063/1.3310930

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A droplet-based micro-total-analysis system involving biosensor performance enhancement by integrated surface-acoustic-wave (SAW) microstreaming is shown. The bioreactor consists of an encapsulated droplet with a biosensor on its periphery, with in situ streaming induced by SAW. This paper highlights the characterization by particle image tracking of the speed distribution inside the droplet. The analyte-biosensor interaction is then evaluated by finite element simulation with different streaming conditions. Calculation of the biosensing enhancement shows an optimum in the biosensor response. These results confirm that the evaluation of the Damkoumlhler and Peclet numbers is of primary importance when designing biosensors enhanced by streaming.

引用

页数：5

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