Protein Modification by Endogenously Generated Lipid Electrophiles: Mitochondria as the Source and Target

被引：29

作者：

Beavers, William N. ^{[1
]}

Rose, Kristie L. ^{[2
,5
]}

Galligan, James J. ^{[2
]}

Mitchener, Michelle M. ^{[1
]}

Rouzer, Carol A. ^{[2
]}

Tallman, Keri A. ^{[1
]}

Lamberson, Connor R. ^{[1
]}

Wang, Xiaojing ^{[4
]}

Hill, Salisha ^{[5
]}

Ivanova, Pavlina T. ^{[3
]}

Brown, H. Alex ^{[2
,3
]}

Zhang, Bing ^{[4
]}

Porter, Ned A. ^{[1
]}

Marnett, Lawrence J. ^{[1
,2
,3
]}

机构：

[1] Vanderbilt Univ, AB Hancock Mem Lab Canc Res, Dept Chem,Vanderbilt Ingram Canc Ctr, Vanderbilt Inst Chem Biol,Vanderbilt Ctr Mol Toxi, Nashville, TN 37232 USA

[2] Vanderbilt Univ, Dept Biochem, Vanderbilt Ingram Canc Ctr, Vanderbilt Inst Chem Biol,Vanderbilt Ctr Mol Toxi, Nashville, TN 37232 USA

[3] Vanderbilt Univ, Dept Pharmacol, Vanderbilt Ingram Canc Ctr, Vanderbilt Inst Chem Biol,Vanderbilt Ctr Mol Toxi, Nashville, TN 37232 USA

[4] Vanderbilt Univ, Dept Biomed Informat, Vanderbilt Ingram Canc Ctr, Vanderbilt Inst Chem Biol,Vanderbilt Ctr Mol Toxi, Nashville, TN 37232 USA

[5] Vanderbilt Univ, Vanderbilt Mass Spectrometry Res Ctr, Vanderbilt Ingram Canc Ctr, Vanderbilt Inst Chem Biol,Vanderbilt Ctr Mol Toxi, Nashville, TN 37232 USA

来源：

ACS CHEMICAL BIOLOGY | 2017年 / 12卷 / 08期

基金：

美国国家卫生研究院;

关键词：

POLYUNSATURATED FATTY-ACIDS; CLICK CHEMISTRY; RAW264.7; CELLS; METABOLISM; LIPOPOLYSACCHARIDE; IDENTIFICATION; INDUCTION; GENE; PEROXIDATION; MACROPHAGES;

D O I：

10.1021/acschembio.7b00480

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Determining the impact of lipid electrophile-mediated protein damage that occurs during oxidative stress requires a comprehensive analysis of electrophile targets adducted under pathophysiological conditions. Incorporation of ?-alkynyl linoleic acid into the phospholipids of macrophages prior to activation by Kdo2-lipid A, followed by protein extraction, click chemistry, and streptavidin affinity capture, enabled a systems-level survey of proteins adducted by lipid electrophiles generated endogenously during the inflammatory response. Results revealed a dramatic enrichment for membrane and mitochondrial proteins as targets for adduction. A marked decrease in adduction in the presence of MitoTEMPO demonstrated a primary role for mitochondrial superoxide in electrophile generation and indicated an important role for mitochondria as both a source and target of lipid electrophiles, a finding that has not been revealed by prior studies using exogenously provided electrophiles.

引用

页码：2062 / 2069

页数：8

共 40 条

[21] Capture and release of alkyne-derivatized glycerophospholipids using cobalt chemistry [J].

Milne, Stephen B. ;

Tallman, Keri A. ;

Serwa, Remigiusz ;

Rouzer, Carol A. ;

Armstrong, Michelle D. ;

Marnett, Lawrence J. ;

Lukehart, Charles M. ;

Porter, Ned A. ;

Brown, H. Alex .

NATURE CHEMICAL BIOLOGY, 2010, 6 (03) :205-207

[22] Nonresolving Inflammation [J].

Nathan, Carl ;

Ding, Aihao .

CELL, 2010, 140 (06) :871-882

[23] Stable isotope labeling by amino acids in cell culture, SILAC, as a simple and accurate approach to expression proteomics [J].

Ong, SE ;

Blagoev, B ;

Kratchmarova, I ;

Kristensen, DB ;

Steen, H ;

Pandey, A ;

Mann, M .

MOLECULAR & CELLULAR PROTEOMICS, 2002, 1 (05) :376-386

[24] A practical recipe for stable isotope labeling by amino acids in cell culture (SILAC) [J].

Ong, Shao-En ;

Mann, Matthias .

NATURE PROTOCOLS, 2006, 1 (06) :2650-2660

[25] Heme Oxygenase-1 Couples Activation of Mitochondrial Biogenesis to Anti-inflammatory Cytokine Expression [J].

Piantadosi, Claude A. ;

Withers, Crystal M. ;

Bartz, Raquel R. ;

MacGarvey, Nancy Chou ;

Fu, Ping ;

Sweeney, Timothy E. ;

Welty-Wolf, Karen E. ;

Suliman, Hagir B. .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2011, 286 (18) :16374-16385

[26] Kdo2-Lipid A of Escherichia coli, a defined endotoxin that activates macrophages via TLR-4 [J].

Raetz, Christian R. H. ;

Garrett, Teresa A. ;

Reynolds, C. Michael ;

Shaw, Walter A. ;

Moore, Jeff D. ;

Smith, Dale C., Jr. ;

Ribeiro, Anthony A. ;

Murphy, Robert C. ;

Ulevitch, Richard J. ;

Fearns, Colleen ;

Reichart, Donna ;

Glass, Christopher K. ;

Benner, Chris ;

Subramaniam, Shankar ;

Harkewicz, Richard ;

Bowers-Gentry, Rebecca C. ;

Buczynski, Matthew W. ;

Cooper, Jennifer A. ;

Deems, Raymond A. ;

Dennis, Edward A. .

JOURNAL OF LIPID RESEARCH, 2006, 47 (05) :1097-1111

[27] On the cellular metabolism of the click chemistry probe 19-alkyne arachidonic acid [J].

Robichaud, Philippe Pierre ;

Poirier, Samuel J. ;

Boudreau, Luc H. ;

Doiron, Jeremie A. ;

Barnett, David A. ;

Boilard, Eric ;

Surette, Marc E. .

JOURNAL OF LIPID RESEARCH, 2016, 57 (10) :1821-1830

[28] Substrate Fate in Activated Macrophages: A Comparison between Innate, Classic, and Alternative Activation [J].

Rodriguez-Prados, Juan-Carlos ;

Traves, Paqui G. ;

Cuenca, Jimena ;

Rico, Daniel ;

Aragones, Julian ;

Martin-Sanz, Paloma ;

Cascante, Marta ;

Bosca, Lisardo .

JOURNAL OF IMMUNOLOGY, 2010, 185 (01) :605-614

[29] RAW264.7 cells lack prostaglandin-dependent autoregulation of tumor necrosis factor-α secretion [J].

Rouzer, CA ;

Jacobs, AT ;

Nirodi, CS ;

Kingsley, PJ ;

Morrow, JD ;

Marnett, LJ .

JOURNAL OF LIPID RESEARCH, 2005, 46 (05) :1027-1037

[30] Mechanism of free radical oxygenation of polyunsaturated fatty acids by cyclooxygenases [J].

Rouzer, CA ;

Marnett, LJ .

CHEMICAL REVIEWS, 2003, 103 (06) :2239-2304

← 1 2 3 4 →