Genetic and metabolic engineering for microbial production of poly-γ-glutamic acid

被引：77
作者：
Cao, Mingfeng [1 ]
Feng, Jun [2 ]
Sirisansaneeyakul, Sarote [3 ]
Song, Cunjiang [4 ]
Chisti, Yusuf [5 ]
机构：
[1] Iowa State Univ, Dept Chem & Biol Engn, NSF Ctr Biorenewable Chem CBiRC, Ames, IA 50011 USA
[2] Auburn Univ, Dept Biosyst Engn, Auburn, AL 36849 USA
[3] Kasetsart Univ, Fac Agroind, Dept Biotechnol, Bangkok 10900, Thailand
[4] Nankai Univ, Key Lab Mol Microbiol & Technol, Minisuy Educ, Tianjin 300071, Peoples R China
[5] Massey Univ, Sch Engn, Private Bag 11 222, Palmerston North, New Zealand
来源：
BIOTECHNOLOGY ADVANCES | 2018年 / 36卷 / 05期
基金：
国家高技术研究发展计划(863计划); 中国国家自然科学基金;
关键词：
Biopolymers; Glutamic acid; Pathway engineering; Poly-gamma-glutamic acid; Recombinant producers; RECOMBINANT ESCHERICHIA-COLI; LICHENIFORMIS TISTR 1010; BACILLUS-SUBTILIS; POLY(GAMMA-GLUTAMIC ACID); PGA; AMYLOLIQUEFACIENS; BIOSYNTHESIS; EXPRESSION; DEGRADATION; INTEGRATION;
D O I：
10.1016/j.biotechadv.2018.05.006
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Poly-gamma-glutamic acid (gamma-PGA) is a natural biopolymer of glutamic acid. The repeating units of gamma-PGA may be derived exclusively from D-glutamic acid, or L-glutamic acid, or both. The monomer units are linked by amide bonds between the alpha-amino group and the gamma-carboxylic acid group. gamma-PGA is biodegradable, edible and water-soluble. It has numerous existing and emerging applications in processing of foods, medicines and cosmetics. This review focuses on microbial production of gamma-PGA via genetically and metabolically engineered recombinant bacteria. Strategies for improving production of gamma-PGA include modification of its biosynthesis pathway, enhancing the production of its precursor (glutamic acid), and preventing loss of the precursor to competing byproducts. These and other strategies are discussed. Heterologous synthesis of gamma-PGA in industrial bacterial hosts that do not naturally produce gamma-PGA is discussed. Emerging trends and the challenges affecting the production of gamma-PGA are reviewed.
引用
收藏
页码：1424 / 1433
页数：10
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