Characterization of a New Defensin from Cowpea (Vigna unguiculata (L.) Walp.)

被引：18

作者：

Padovan, Lara ^{[1
,2
,3
]}

Segat, Ludovica ^{[1
,2
,4
]}

Tossi, Alessandro ^{[3
]}

Calsa, Tercilio, Jr. ^{[5
]}

Ederson, Akio Kido ^{[5
]}

Brandao, Lucas ^{[4
]}

Guimaraes, Rafael L. ^{[4
]}

Pandolfi, Valesca ^{[5
]}

Pestana-Calsa, Maria Clara ^{[5
]}

Belarmino, Luis Carlos ^{[5
]}

Benko-Iseppon, Ana Maria ^{[5
]}

Crovella, Sergio ^{[1
,2
,5
]}

机构：

[1] IRCCS Burlo Garofolo, Genet Serv, I-34137 Trieste, Italy

[2] Univ Trieste, Dept Dev & Reprod Sci, I-34137 Trieste, Italy

[3] Univ Trieste, Dept Life Sci, I-34127 Trieste, Italy

[4] Univ Fed Pernambuco, LIKA, BR-50670901 Recife, PE, Brazil

[5] Univ Fed Pernambuco, Ctr Ciencias Biol, Dept Genet, BR-50670901 Recife, PE, Brazil

来源：

PROTEIN AND PEPTIDE LETTERS | 2010年 / 17卷 / 03期

关键词：

Antimicrobial peptides; cowpea mosaic virus; evolution; plant defensin; protein function; Vigna unguiculata; PLANT-DEFENSIN; GAMMA-THIONIN; PEPTIDES; EXPRESSION; PROTEIN; CDNA; GENE; INHIBITORS; SEQUENCE; INSIGHTS;

D O I：

10.2174/092986610790780350

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Using Phaseoleae defensins available in databases, a putative defensin gene was isolated in cowpea (Vigna unguiculata (L.) Walp.) and cloned from genomic cowpea DNA. The putative mature defensin sequence displays the characteristic defensins residues arrangement, secondary and tertiary structures were predicted and splicing analysis was performed. Using RT-PCR, defensin expression and differences in response to biotic stimuli between infected and non infected plants were tested.

引用

页码：297 / 304

页数：8

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