Application and field validation of a PCR assay for the detection of Mycoplasma hyopneumoniae from swine lung tissue samples

被引：18

作者：

Cai, Hugh Y. ^{[1
]}

van Dreumel, Tony ^{[1
]}

McEwen, Beverly ^{[1
]}

Hornby, Geoff ^{[1
]}

Bell-Rogers, Patricia ^{[1
]}

McRaild, Pat ^{[1
]}

Josephson, Gaylan ^{[1
]}

Maxie, Grant ^{[1
]}

机构：

[1] Univ Guelph, Hlth Anim Lab, Lab Serv Div, Guelph, ON N1H 6R8, Canada

来源：

JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION | 2007年 / 19卷 / 01期

关键词：

Bayesian modeling; enzootic pneumonia; FA test; Mycoplasma hyopneunioniae; PCR;

D O I：

10.1177/104063870701900115

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

A PCR assay was validated for the detection of Mycoplasma hyopneumoniae in porcine lung tissue. The detection limit of the assay was 0.18 colony-forming units/g of lung sample spiked with M. hyopneumoniae. In field validation, 426 pigs from 220 cases were examined for M. hyopneumoniae infection by M. hyopneumoniae PCR and a fluorescent antibody (FA) test. In total, 103 pig lungs (24.2%) were positive in the PCR test, and 69 pig lungs (16.2%) were positive in the FA test, among which, 62 pigs were positive for both PCR and FA test. Most of the PCR-positive but FA test-negative cases had lesions compatible with M. hyopneumoniae infection. With Bayesian modeling, the diagnostic sensitivity and specificity of the PCR were determined to be 97.3% and 93.0%, respectively.

引用

页码：91 / 95

页数：5

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