Bessel-beam illumination Raman microscopy

被引：14

作者：

Bando, Kazuki ^{[1
]}

Yabuuchi, Shumpei ^{[1
]}

LI, Menglu ^{[1
,2
]}

Kubo, Toshiki ^{[1
]}

Oketani, Ryosuke ^{[1
]}

Smith, Nicholas I. ^{[3
]}

Fujita, Katsumasa ^{[1
,2
,4
]}

机构：

[1] Osaka Univ, Dept Appl Phys, 2-1 Yamadaoka, Suita, Osaka 5650871, Japan

[2] Natl Inst Adv Ind Sci & Technol, AIST Osaka Univ Adv Photon & Biosensing Open Inno, 2-1 Yamadaoka, Suita, Osaka 5650871, Japan

[3] Osaka Univ, Immunol Frontier Res Ctr, 2-1 Yamadaoka, Suita, Osaka 5650871, Japan

[4] Osaka Univ, Inst Open & Transdisciplinary Res Initiat, 2-1 Yamadaoka, Suita, Osaka 5650871, Japan

来源：

BIOMEDICAL OPTICS EXPRESS | 2022年 / 13卷 / 06期

关键词：

FLUORESCENCE; CULTURE; FIELD;

D O I：

10.1364/BOE.456138

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We demonstrate the use of Bessel beams for side illumination slit-scanning Raman imaging for label-free and hyperspectral analysis of cell spheroids. The background elimination by the side illumination and the aberration-resistant Bessel beam drastically improves the image contrast in Raman observation, allowing label-free investigation of intracellular molecules in thick biological samples. Live cell spheroids were observed to confirm the improvement in image contrast and background reduction with Bessel illumination compared to conventional epi-line illumination.

引用

页码：3161 / 3170

页数：10

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