Cytomegalovirus infection alters phenotypes of different γδ T-cell subsets in renal transplant recipients with long-term stable graft function

Cytomegalovirus (CMV) infection alters the phenotypic profiles of T-cells and NK cells in healthy and immunocompromised individuals. Here, we examined the effects of CMV infection on the phenotype and functions gamma delta T-cell subsets in renal transplant recipients (RTR) stable several years after transplantation (n = 80) and healthy controls (n = 72). Differentiation status, function, and expression of HLA-DR, CD57, and LIR-1 on V delta 2(-) and V delta 2(+) gamma delta T-cells were examined in peripheral blood cells using flow cytometry. Percentages of V delta 2(-) gamma delta T-cells were higher in RTR who are CMV-seropositive and correlated with CMV antibody levels. Proportions of V delta 2(-) gamma delta T-cells expressing HLA-DR, CD57, or LIR-1 were increased in CMV-seropositive RTR and healthy controls compared to their seronegative counterparts. Additionally, V delta 2(-)gamma delta T-cells were skewed towards a terminally differentiated phenotype and most expressed CD8 in individuals who were CMV-seropositive. Increased expression of LIR-1 on terminally differentiated V delta 2(-) gamma delta T-cells was associated with CMV seropositivity in RTR and controls. The presence of CMV DNA in 15 RTR was associated with higher frequencies of LIR-1+ V delta 2(+) gamma delta T-cells and increased percentages of terminally differentiated effector memory cells in T-cell subsets. Our study further characterises the effects of CMVand transplantation on gamma delta T-cell phenotypes.