Characterizing the Genomic Landscape of Brain Glioma With Circulating Tumor DNA From Tumor In Situ Fluid

被引：12

作者：

Sheng, Zhiyuan ^{[1
]}

Yu, Jinliang ^{[1
]}

Deng, Kaiyuan ^{[1
]}

Andrade-Barazarte, Hugo ^{[1
]}

Zemmar, Ajmal ^{[1
]}

Li, Sijia ^{[3
]}

Li, Nianxuan ^{[2
]}

Yan, Zhaoyue ^{[1
]}

Chen, Zhongcan ^{[1
]}

Sun, Yong ^{[1
]}

Hernesniemi, Juha ^{[1
]}

Bu, Xingyao ^{[1
]}

机构：

[1] Zhengzhou Univ, Henan Prov Peoples Hosp, Dept Neurosurg, Peoples Hosp, Zhengzhou, Peoples R China

[2] Henan Univ Chinese Med, Sch Basic Med, Zhengzhou, Peoples R China

[3] Zhengzhou Univ, Dept Neurol, Affiliated Hosp 5, Zhengzhou, Peoples R China

来源：

FRONTIERS IN ONCOLOGY | 2021年 / 11卷

关键词：

tumor in situ fluid; circulating tumor DNA; glioma; precision medicine; liquid biopsy;

D O I：

10.3389/fonc.2021.584988

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Tumor in situ fluid (TISF) refers to the fluid at the local surgical cavity. We evaluated the feasibility of TISF-derived circulating tumor DNA (ctDNA) characterizing the genomic landscape for glioma. This retrospective study included TISF and tumor samples from 10 patients with glioma, we extracted cell-free DNA (cfDNA) from the TISF and then performed deep sequencing on that. And we compared genomic alterations between TISF and tumor tissue. Results showed that the concentration of cfDNA fragments from the patients for TISF ranged from 7.2 to 1,397 ng/ml. At least one tumor-specific mutation was identified in all 10 patients (100%). Further analysis of TISF ctDNA revealed a broad spectrum of genetic mutations, which have been reported to have clinical relevance. The analysis of concordance between TISF and tumor tissue reflected the spatiotemporal heterogeneity of glioma. Collectively, TISF ctDNA was a powerfully potential source for characterizing the genomic landscape of glioma, which provided new possibilities for precision medicine in patients with glioma.

引用

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