Molecular cloning and characterization of CMCase gene (celC) from Salmonella typhimurium UR

被引：0
作者：
Yoo, JS
Jung, YJ
Chung, SY
Lee, YC
Choi, YL [1 ]
机构：
[1] Dong A Univ, Fac Nat Resources & Life Sci, Div Biotechnol, Pusan 604714, South Korea
[2] Dongju Coll, Dept Food Sci & Nutr, Pusan, South Korea
来源：
JOURNAL OF MICROBIOLOGY | 2004年 / 42卷 / 03期
关键词：
Salmonella typhimurium; CMCase; celC; CMC-SDS-PAGE;
D O I：
暂无
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The sequence coding for carboxymethylcellulase (CMCase, CelC) was isolated from the DNA of Salmonella typhimurium UR1. Comparison between the deduced amino acid sequence of CelC (368 amino acid residues, Molecular mass 41 kDa) and that of the previously published CMCase revealed that this enzyme belongs to the cellulase family 8 and A The protein was overproduced in Escherichia coli using T7 expression system, and its activity was confirmed by CMC-SDS-PAGE. When the overexpressed CelC protein was tested on cellulose-type substrates, the recombinant protein is able to degrade cellulose-type substrates, such as CM-cellulose, xylan, avicel, lichenan, and laminarin. Optimal temperature and pH for enzyme activity were found to be 50degreesC and pH 6.5, respectively.
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页码：205 / 210
页数：6
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