Detection and Characteristics of Rifampicin-Resistant Isolates of Mycobacterium tuberculosis

被引：2

作者：

Cherednichenko, A. G. ^{[1
]}

Dymova, M. A. ^{[2
,3
]}

Solodilova, O. A. ^{[4
]}

Petrenko, T. I. ^{[1
]}

Prozorov, A. I. ^{[4
]}

Filipenko, M. L. ^{[2
,3
]}

机构：

[1] Minist Hlth Russian Federat, Res Inst TB, Novosibirsk, Russia

[2] Russian Acad Sci, Siberian Div, Inst Chem Biol & Fundamental Med, Novosibirsk, Russia

[3] Novosibirsk State Univ, Novosibirsk 630090, Russia

[4] Altai Reg AntiTB Dispensary, Barnaul, Russia

来源：

BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE | 2016年 / 160卷 / 05期

关键词：

Mycobacterium tuberculosis; drug resistance; rifampicin; rpoB gene;

D O I：

10.1007/s10517-016-3243-3

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Genotyping and analysis the drug resistance of 59 isolates of M. tuberculosis obtained from patients living in Altai Territory were performed using a BACTEC MGIT 960 fluorometric system by means of VNTR typing (variable number tandem repeat), PCR-RFLP analysis, and sequence analysis. The occurrence frequency was highest for isolates of the Beijing family (n=30, 50.8%). Analysis of mutation spectrum in the rpoB gene associated with rifampicin resistance revealed the major mutation (codon 531 of the rpoB gene) in 93% samples, which allows us to use rapid test systems.

引用

页码：659 / 663

页数：5

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