Green Synthesized Silver Nanoparticles Using Lactobacillus Acidophilus as an Antioxidant, Antimicrobial, and Antibiofilm Agent Against Multi-drug Resistant Enteroaggregative Escherichia Coli

被引：45

作者：

Abishad, Padikkamannil ^{[1
]}

Vergis, Jess ^{[1
]}

Unni, Varsha ^{[1
]}

Ram, Vemula Prasastha ^{[2
]}

Niveditha, Pollumahanti ^{[2
]}

Yasur, Jyothsana ^{[2
]}

Juliet, Sanis ^{[3
]}

John, Lijo ^{[4
]}

Byrappa, Kullaiya ^{[5
]}

Nambiar, Prejit ^{[1
]}

Kurkure, Nitin Vasantrao ^{[6
]}

Barbuddhe, Sukhadeo Baliram ^{[2
]}

Rawool, Deepak Bhiwa ^{[2
]}

机构：

[1] Kerala Vet & Anim Sci Univ, Coll Vet & Anim Sci, Dept Vet Publ Hlth, Lakkidi Post 673576, Wayanad, India

[2] ICAR Natl Res Ctr Meat, Hyderabad 500092, India

[3] Kerala Vet & Anim Sci Univ, Coll Vet & Anim Sci, Dept Vet Pharmacol & Toxicol, Lakkidi Post 673576, Wayanad, India

[4] Kerala Vet & Anim Sci Univ, Coll Vet & Anim Sci, Dept Vet Biochem, Lakkidi Post 673576, Wayanad, India

[5] Adichunchanagiri Univ, Ctr Res & Innovat, BGSIT, Bg Mandya 571418, India

[6] Maharashtra Anim & Fishery Sci Univ, Nagpur Vet Coll, Dept Vet Pathol, Nagpur 440006, Maharashtra, India

来源：

PROBIOTICS AND ANTIMICROBIAL PROTEINS | 2022年 / 14卷 / 05期

关键词：

Antimicrobial resistance; Enteroaggregative Escherichia coli; Green synthesis; Lactobacillus acidophilus; Nanoparticles; Silver; ZNO NANOPARTICLES; SURFACE;

D O I：

10.1007/s12602-022-09961-1

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The present study was envisaged to employ the green synthesis and characterization of silver nanoparticles (AgNPs) using the potential probiotic strain Lactobacillus acidophilus, to assess its antibacterial as well as antibiofilm activity against multi-drug-resistant enteroaggregative Escherichia coli (MDR-EAEC) strains and to investigate their antioxidant activity. In this study, AgNPs were successfully synthesized through an eco-friendly protocol, which was then confirmed by its X-ray diffraction (XRD) pattern. A weight loss of 15% up to 182 degrees C with a narrow exothermic peak between 170 degrees C and 205 degrees C was observed in thermogravimetric analysis-differential thermal analysis (TGA-DTA), while aggregated nanoclusters were observed in scanning electron microscopy (SEM). Moreover, the transmission electron microscopy (TEM) imaging of AgNPs revealed a spherical morphology and crystalline nature with an optimum size ranging from 10 to 20 nm. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values of green synthesized AgNPs against the MDR-EAEC strains were found to be 7.80 mg/L and 15.60 mg/L, respectively. In vitro time-kill kinetic assay revealed a complete elimination of the MDR-EAEC strains after 180 min on co-incubation with the AgNPs. Moreover, the green synthesized AgNPs were found safe by in vitro haemolytic assay. Besides, the green synthesized AgNPs exhibited significant biofilm inhibition (P < 0.001) formed by MDR-EAEC strains. Additionally, a concentration-dependent antioxidant activity was observed in 2,2 '-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assays. Hence, this study demonstrated potential antibacterial as well as antibiofilm activity of green synthesized AgNPs against MDR-EAEC strains with antioxidant properties and warrants further in-depth studies to explore it as an effective antimicrobial agent against MDR infections.

引用

页码：904 / 914

页数：11

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