An Improved Protocol for the Preparation of Total Genomic DNA from Isolates of Yeast and Mould Using Whatman FTA Filter Papers

被引：24

作者：

Borman, Andrew M. ^{[1
,2
]}

Fraser, Mark ^{[2
]}

Linton, Christopher J. ^{[2
]}

Palmer, Michael D. ^{[2
]}

Johnson, Elizabeth M. ^{[2
]}

机构：

[1] HPA Mycol Reference Lab, Bristol BS2 8EL, Avon, England

[2] Hlth Protect Agcy, SW Reg Lab, Mycol Reference Lab, Bristol, Avon, England

来源：

MYCOPATHOLOGIA | 2010年 / 169卷 / 06期

关键词：

Fungal genomic DNA; FTA technology; PCR; DNA extraction; Molecular identification; SPECIES COMPLEX; PSEUDALLESCHERIA; FUSARIUM;

D O I：

10.1007/s11046-010-9284-7

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Here, we present a significantly improved version of our previously published method for the extraction of fungal genomic DNA from pure cultures using Whatman FTA(R) filter paper matrix technology. This modified protocol is extremely rapid, significantly more cost effective than our original method, and importantly, substantially reduces the problem of potential cross-contamination between sequential filters when employing FTA technology.

引用

页码：445 / 449

页数：5

共 11 条

[1] Sequence-Based Identification of Aspergillus, Fusarium, and Mucorales Species in the Clinical Mycology Laboratory: Where Are We and Where Should We Go from Here? [J].

Balajee, S. A. ;

Borman, A. M. ;

Brandt, M. E. ;

Cano, J. ;

Cuenca-Estrella, M. ;

Dannaoui, E. ;

Guarro, J. ;

Haase, G. ;

Kibbler, C. C. ;

Meyer, W. ;

O'Donnell, K. ;

Petti, C. A. ;

Rodriguez-Tudela, J. L. ;

Sutton, D. ;

Velegraki, A. ;

Wickes, B. L. .

JOURNAL OF CLINICAL MICROBIOLOGY, 2009, 47 (04) :877-884

[2] Molecular Identification of Aspergillus Species Collected for the Transplant-Associated Infection Surveillance Network [J].

Balajee, S. Arunmozhi ;

Kano, Rui ;

Baddley, John W. ;

Moser, Stephen A. ;

Marr, Kieren A. ;

Alexander, Barbara D. ;

Andes, David ;

Kontoyiannis, Dimitrios P. ;

Perrone, Giancarlo ;

Peterson, Stephen ;

Brandt, Mary E. ;

Pappas, Peter G. ;

Chiller, Tom .

JOURNAL OF CLINICAL MICROBIOLOGY, 2009, 47 (10) :3138-3141

[3] Ultra-rapid preparation of total genomic DNA from isolates of yeast and mould using Whatman FTA filter paper technology - a reusable DNA archiving system [J].

Borman, Andrew M. ;

Linton, Christopher J. ;

Miles, Sarah-Jane ;

Campbell, Colin K. ;

Johnson, Elizabeth M. .

MEDICAL MYCOLOGY, 2006, 44 (05) :389-398

[4]

Clinical and Laboratory Standards Institute, 2008, MM18A CLSI

[5] Cryptic species in Stachybotrys chartarum [J].

Cruse, M ;

Telerant, R ;

Gallagher, T ;

Lee, T ;

Taylor, JW .

MYCOLOGIA, 2002, 94 (05) :814-822

[6] Molecular phylogeny of the Pseudallescheria boydii species complex:: Proposal of two new species [J].

Gilgado, F ;

Cano, J ;

Gené, J ;

Guarro, J .

JOURNAL OF CLINICAL MICROBIOLOGY, 2005, 43 (10) :4930-4942

[7]

Issakainen J, 1997, J MED VET MYCOL, V35, P389

[8] Emerging fungal diseases [J].

Nucci, M ;

Marr, KA .

CLINICAL INFECTIOUS DISEASES, 2005, 41 (04) :521-526

[9] Molecular phylogenetic diversity, multilocus haplotype nomenclature, and in vitro antifungal resistance within the Fusarium solani species complex [J].

O'Donnell, Kerry ;

Sutton, Deanna A. ;

Fothergill, Annette ;

McCarthy, Dora ;

Rinaldi, Michael G. ;

Brandt, Mary E. ;

Zhang, Ning ;

Geiser, David M. .

JOURNAL OF CLINICAL MICROBIOLOGY, 2008, 46 (08) :2477-2490

[10] Changing epidemiology of systemic fungal infections [J].

Richardson, M. ;

Lass-Floerl, C. .

CLINICAL MICROBIOLOGY AND INFECTION, 2008, 14 :5-24

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