Sensitive bioanalysis -: combining single-molecule spectroscopy with mono-labeled self-quenching probes

被引：17
作者：
Marme, Nicole
Knemeyer, Jens-Peter
机构：
[1] Heidelberg Univ, Inst Chem Phys, D-69120 Heidelberg, Germany
[2] German Canc Res Ctr, Dept Funct Genome Anal, D-69120 Heidelberg, Germany
来源：
ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2007年 / 388卷 / 5-6期
关键词：
single-molecule spectroscopy; photoinduced electron transfer (PET); self-quenching probes; ultra-sensitive bioanalysis;
D O I：
10.1007/s00216-007-1365-1
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Fluorescence single-molecule spectroscopy is an appropriate tool for modern bioanalysis. This technique enables the development of ultra sensitive assays, especially when combined with self-quenching probes. In this review we report novel DNA, enzyme, and antibody assays based on mono-labeled fluorescent probes that are quenched by photoinduced electron transfer (PET).
引用
收藏
页码：1075 / 1085
页数：11
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