Detection and identification of 4-hydroxy-2-nonenal Schiff-base adducts along with products of Michael addition using data-dependent neutral loss-driven MS3 acquisition: Method evaluation through an in vitro study on cytochrome c oxidase modifications

被引：37

作者：

Rauniyar, Navin ^{[1
]}

Prokai, Laszlo ^{[1
]}

机构：

[1] Univ N Texas Hlth Sci Ctr, Dept Mol Biol & Immunol, Ft Worth, TX 76107 USA

来源：

PROTEOMICS | 2009年 / 9卷 / 22期

基金：

美国国家卫生研究院;

关键词：

4-Hydroxy-2-nonenal; Michael addition; Neutral loss-driven MS3; Protein adducts; Schiff base; Technology; TANDEM MASS-SPECTROMETRY; STATISTICAL-MODEL; PROTEIN; CHEMISTRY; SUBUNITS; HEART;

D O I：

10.1002/pmic.200900116

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We report a data-dependent neutral-loss-driven MS3 acquisition to enhance, in addition to abundant Michael adducts, the detection of Schiff-base adducts of proteins and 4-hydroxy-2-nonenal, a reactive end product of lipid peroxidation. In vitro modification of cytochrome c oxidase, a mitochondrial protein complex, was used as a model to evaluate the method. The technique allowed for a confident validation of modification sites and also identified a Schiff-base adduct in subunit Vb of the protein complex.

引用

页码：5188 / 5193

页数：6

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