DEVELOPMENT OF MICROSATELLITE LOCI IN MEDITERRANEAN SARSAPARILLA (SMILAX ASPERA; SMILACACEAE) USING TRANSCRIPTOME DATA

被引：10

作者：

Qi, Zhe-Chen ^{[1
,2
]}

Shen, Chao ^{[1
,2
]}

Han, Yu-Wei ^{[1
]}

Shen, Wei

Yang, Man ^{[1
]}

Liu, Jinliang ^{[1
]}

Liang, Zong-Suo ^{[1
,2
]}

Li, Pan ^{[3
,4
]}

Fu, Cheng-Xin ^{[3
,4
]}

机构：

[1] Zhejiang Sci Tech Univ, Coll Life Sci, Hangzhou 310018, Zhejiang, Peoples R China

[2] Zhejiang Prov Key Lab Plant Secondary Metab & Re, Hangzhou 310018, Zhejiang, Peoples R China

[3] Zhejiang Univ, Minist Educ, Coll Life Sci, Key Lab Conservat Biol Endangered Wildlife, Hangzhou 310058, Zhejiang, Peoples R China

[4] Zhejiang Univ, Coll Life Sci, Lab Systemat & Evolutionary Bot & Biodivers, Hangzhou 310058, Zhejiang, Peoples R China

来源：

APPLICATIONS IN PLANT SCIENCES | 2017年 / 5卷 / 04期

基金：

中国国家自然科学基金;

关键词：

deep lineage divergence; intercontinental disjunction; microsatellites; Smilacaceae; Smilax aspera; Tethyan vegetation; transcriptome; SOFTWARE;

D O I：

10.3732/apps.1700005

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Premise of the study: Although several microsatellite markers of Smilax aspera (Smilacaceae) have been reported in a previous study, due to universality issues in cross-population amplification, we have newly developed microsatellite markers for S. aspera based on transcriptome data to further investigate gene flow and genetic structure of its circum-Mediterranean, East African, and South Asian populations. Methods and Results: A total of 4854 simple sequence repeat (SSR) primer pairs were designed from 99,193 contigs acquired from public transcriptome data of S. bona-nox. Forty-six microsatellite loci were selected for further genotyping in 12 S. aspera populations. The number of alleles varied from three to 28, and 93.5% of the developed microsatellite markers could be cross-amplified in least one of three congeneric Smilax species. Conclusions: The SSR markers developed in this study will facilitate further studies on genetic diversity and phylogeographic patterns of S. aspera in intercontinental geographical scales.

引用

页数：6

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