Sensitivity enhancement by exchange mediated magnetization transfer of the xenon biosensor signal

被引：23
作者：
Garcia, Sandra
Chavez, Lana
Lowery, Thomas J.
Han, Song-I
Wemmer, David E.
Pines, Alexander [1 ]
机构：
[1] Lawrence Berkeley Lab, Div Sci Mat, Berkeley, CA USA
[2] Lawrence Berkeley Lab, Phys Biosci Div, Berkeley, CA USA
[3] Univ Calif Berkeley, Dept Chem, Berkeley, CA 94720 USA
[4] Univ Calif Santa Barbara, Dept Chem, Santa Barbara, CA 93106 USA
来源：
JOURNAL OF MAGNETIC RESONANCE | 2007年 / 184卷 / 01期
关键词：
xenon; biosensor; remote detection; chemical exchange;
D O I：
10.1016/j.jmr.2006.09.010
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Hyperpolarized xenon associated with ligand derivatized cryptophane-A cages has been developed as a NMR based biosensor. To optimize the detection sensitivity we describe use of xenon exchange between the caged and bulk dissolved xenon as an effective signal amplifier. This approach, somewhat analogous to 'remote detection' described recently, uses the chemical exchange to repeatedly transfer spectroscopic information from caged to bulk xenon, effectively integrating the caged signal. After an optimized integration period, the signal is read out by observation of the bulk magnetization. The spectrum of the caged xenon is reconstructed through use of a variable evolution period before transfer and Fourier analysis of the bulk signal as a function of the evolution time. (c) 2006 Elsevier Inc. All rights reserved.
引用
收藏
页码：72 / 77
页数：6
相关论文
共 19 条
[1]   Measuring surface-area-to-volume ratios in soft porous materials using laser-polarized xenon interphase exchange nuclear magnetic resonance [J].
Butler, JP ;
Mair, RW ;
Hoffmann, D ;
Hrovat, MI ;
Rogers, RA ;
Topulos, GP ;
Walsworth, RL ;
Patz, S .
JOURNAL OF PHYSICS-CONDENSED MATTER, 2002, 14 (13) :L297-L304
[2]   Nuclear magnetic resonance of laser-polarized noble gases in molecules, materials, and organisms [J].
Goodson, BM .
JOURNAL OF MAGNETIC RESONANCE, 2002, 155 (02) :157-216
[3]   Sensitivity quantification of remote detection NMR and MRI [J].
Granwehr, J ;
Seeley, JA .
JOURNAL OF MAGNETIC RESONANCE, 2006, 179 (02) :280-289
[4]   Detection of proton chemical exchange between metabolites and water in biological tissues [J].
Guivel-Scharen, V ;
Sinnwell, T ;
Wolff, SD ;
Balaban, RS .
JOURNAL OF MAGNETIC RESONANCE, 1998, 133 (01) :36-45
[5]   NMR-based biosensing with optimized delivery of polarized 129Xe to solutions [J].
Han, SI ;
Garcia, S ;
Lowery, TJ ;
Ruiz, EJ ;
Seeley, JA ;
Chavez, L ;
King, DS ;
Wemmer, DE ;
Pines, A .
ANALYTICAL CHEMISTRY, 2005, 77 (13) :4008-4012
[6]   Spectrally resolved magnetic resonance imaging of a xenon biosensor [J].
Hilty, C ;
Lowery, TJ ;
Wemmer, DE ;
Pines, A .
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2006, 45 (01) :70-73
[7]   Probing proteins in solution by 129Xe NMR spectroscopy [J].
Locci, E ;
Dehouck, Y ;
Casu, M ;
Saba, G ;
Lai, A ;
Luhmer, M ;
Reisse, J ;
Bartik, K .
JOURNAL OF MAGNETIC RESONANCE, 2001, 150 (02) :167-174
[8]   Optimization of xenon biosensors for detection of protein interactions [J].
Lowery, TJ ;
Garcia, S ;
Chavez, L ;
Ruiz, EJ ;
Wu, T ;
Brotin, T ;
Dutasta, JP ;
King, DS ;
Schultz, PG ;
Pines, A ;
Wemmer, DE .
CHEMBIOCHEM, 2006, 7 (01) :65-73
[9]   Design of a conformation-sensitive xenon-binding cavity in the ribose-binding protein [J].
Lowery, TJ ;
Rubin, SM ;
Ruiz, EJ ;
Pines, A ;
Wemmer, DE .
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2004, 43 (46) :6320-6322
[10]  
Lowery TJ, 2005, PROTEIN SCI, V14, P848, DOI [10.1110/ps.041231005, 10.1110/ps.041231005.]
12