Aberrant Methylation of Tumor Suppressive miRNAs in Bile from Patients With Pancreaticobiliary Diseases

被引：8

作者：

Ohtsubo, Koushiro ^{[1
]}

Miyake, Kunio ^{[2
]}

Arai, Sachiko ^{[1
]}

Fukuda, Koji ^{[1
]}

Yanagimura, Naohiro ^{[1
]}

Suzuki, Chiaki ^{[1
]}

Otani, Sakiko ^{[1
]}

Adachi, Yuta ^{[1
]}

Tanimoto, Azusa ^{[1
]}

Nishiyama, Akihiro ^{[1
]}

Yamashita, Kaname ^{[1
]}

Takeuchi, Shinji ^{[1
]}

Notohara, Kenji ^{[3
]}

Yoshimura, Kenichi ^{[4
]}

Yano, Seiji ^{[1
]}

机构：

[1] Kanazawa Univ, Canc Res Inst, Div Med Oncol, 13-1 Takaramachi, Kanazawa, Ishikawa 9200934, Japan

[2] Univ Yamanashi, Sch Med, Dept Hlth Sci, Chuo, Japan

[3] Kurashiki Cent Hosp, Dept Anat Pathol, Kurashiki, Okayama, Japan

[4] Kanazawa Univ, Innovat Clin Res Ctr, Dept Biostat, Kanazawa, Ishikawa, Japan

来源：

ANTICANCER RESEARCH | 2019年 / 39卷 / 10期

基金：

日本学术振兴会;

关键词：

miRNA; methylation; bile; pancreatic cancer; biliary tract cancer; DNA METHYLATION; PROMOTER HYPERMETHYLATION; HUMAN CHOLANGIOCARCINOMA; DOWN-REGULATION; RAS MUTATIONS; CANCER; EXPRESSION; MICRORNAS; BIOMARKERS; GENE;

D O I：

10.21873/anticanres.13738

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Background/Aim: Epigenetic abnormalities in microRNAs (miRNAs) have not been analyzed in samples other than pancreaticobiliary tissues in patients with pancreaticobiliary cancer (PBC). To identify miRNAs specific for PBC, the present study analyzed the methylation of tumor-suppressive miRNAs in bile from patients with pancreaticobiliary diseases. Materials and Methods: Bile was collected endoscopically or percutaneously from 52 patients with pancreatic cancer, 26 with biliary tract cancer, and 20 with benign pancreaticobiliary diseases. Sequences encoding 16 tumor-suppressive miRNAs were amplified by polymerase chain reaction and sequenced, and their methylation rates were determined. Results: The methylation rates of miR-1247 and miR-200a were significantly higher in patients with pancreatic cancer, and biliary tract cancer than in those with benign diseases, and the methylation rate of miR-200b was significantly higher in patients with pancreatic cancer than in those with benign diseases. Conclusion: Methylation of miR-1247, miR-200a, and miR-200b in bile may be useful for distinguishing PBC from benign diseases.

引用

页码：5449 / 5459

页数：11

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