Transformation system for prototrophic industrial yeasts using the AUR1 gene as a dominant selection marker

被引：43

作者：

Hashida-Okado, T ^{[1
]}

Ogawa, A ^{[1
]}

Kato, I ^{[1
]}

Takesako, K ^{[1
]}

机构：

[1] Takara Shuzo Co Ltd, Biotechnol Res Labs, Otsu, Shiga 52021, Japan

来源：

FEBS LETTERS | 1998年 / 425卷 / 01期

关键词：

AUR1-C; transformation system; aureobasidin A; drug resistance; gene disruption; industrial yeast;

D O I：

10.1016/S0014-5793(98)00211-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We show a new transformation system for prototrophic yeast strains including those of Saccharomyces cerevisiae, Kluyveromyces lactis, K. marxianus, and Candida glabrata. This system is composed of an antibiotic, aureobasidin A (AbA), and its resistance gene AUR1-C as a selection marker, Southern analysis of genomic DNAs of the transformants indicated that the cop., number of the plasmid increased from one to more than four, depending on the concentration of AbA used for selection of the transformants. The AUR1-C gene was also effective as a selection marker for gene disruption, and was able to disrupt both copies of the gene on homologous chromosomes of diploid cells by a single round of transformation. This system has a broad application in the transformation and gene disruption of prototrophic strains of a variety of yeast species. (C) 1998 Federation of European Biochemical Societies.

引用

页码：117 / 122

页数：6

共 18 条

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